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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dioscorea elephantipes 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP406492DIC
CSB-EP406492DIC
CSB-BP406492DIC
CSB-MP406492DIC
CSB-EP406492DIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dioscorea elephantipes 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP406493DIC
CSB-EP406493DIC
CSB-BP406493DIC
CSB-MP406493DIC
CSB-EP406493DIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dioscorea elephantipes NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP406494DIC1
CSB-EP406494DIC1
CSB-BP406494DIC1
CSB-MP406494DIC1
CSB-EP406494DIC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP406495ILI
CSB-EP406495ILI
CSB-BP406495ILI
CSB-MP406495ILI
CSB-EP406495ILI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP406496ILI
CSB-EP406496ILI
CSB-BP406496ILI
CSB-MP406496ILI
CSB-EP406496ILI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum Apocytochrome f (petA), partial CSB-YP406497ILI1
CSB-EP406497ILI1
CSB-BP406497ILI1
CSB-MP406497ILI1
CSB-EP406497ILI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP406498ILI
CSB-EP406498ILI
CSB-BP406498ILI
CSB-MP406498ILI
CSB-EP406498ILI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum Protein ycf2 (ycf2), partial CSB-YP406499ILI
CSB-EP406499ILI
CSB-BP406499ILI
CSB-MP406499ILI
CSB-EP406499ILI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP406500ILI1
CSB-EP406500ILI1
CSB-BP406500ILI1
CSB-MP406500ILI1
CSB-EP406500ILI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Illicium oligandrum Putative membrane protein ycf1 (ycf1), partial CSB-YP406501ILI
CSB-EP406501ILI
CSB-BP406501ILI
CSB-MP406501ILI
CSB-EP406501ILI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina Photosystem I iron-sulfur center (psaC) CSB-YP406502RIK
CSB-EP406502RIK
CSB-BP406502RIK
CSB-MP406502RIK
CSB-EP406502RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina ATP synthase subunit delta, chloroplastic (atpD) CSB-YP406503RIK
CSB-EP406503RIK
CSB-BP406503RIK
CSB-MP406503RIK
CSB-EP406503RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP406504RIK1
CSB-EP406504RIK1
CSB-BP406504RIK1
CSB-MP406504RIK1
CSB-EP406504RIK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP406505RIK
CSB-EP406505RIK
CSB-BP406505RIK
CSB-MP406505RIK
CSB-EP406505RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina Translation initiation factor IF-2, chloroplastic (infB), partial CSB-YP406506RIK
CSB-EP406506RIK
CSB-BP406506RIK
CSB-MP406506RIK
CSB-EP406506RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP406507RIK
CSB-EP406507RIK
CSB-BP406507RIK
CSB-MP406507RIK
CSB-EP406507RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP406508RIK
CSB-EP406508RIK
CSB-BP406508RIK
CSB-MP406508RIK
CSB-EP406508RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP406509RIK
CSB-EP406509RIK
CSB-BP406509RIK
CSB-MP406509RIK
CSB-EP406509RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crinia riparia Riparin-1.6 CSB-YP406510CXJ
CSB-EP406510CXJ
CSB-BP406510CXJ
CSB-MP406510CXJ
CSB-EP406510CXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative golgin subfamily A member 8I (GOLGA8IP) CSB-YP406511HU
CSB-EP406511HU
CSB-BP406511HU
CSB-MP406511HU
CSB-EP406511HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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