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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Psychrobacter sp. Adenylosuccinate synthetase (purA) CSB-YP407172PZR
CSB-EP407172PZR
CSB-BP407172PZR
CSB-MP407172PZR
CSB-EP407172PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP407173PZR
CSB-EP407173PZR
CSB-BP407173PZR
CSB-MP407173PZR
CSB-EP407173PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP407174PZR
CSB-EP407174PZR
CSB-BP407174PZR
CSB-MP407174PZR
CSB-EP407174PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Biotin synthase (bioB) CSB-YP407175PZR
CSB-EP407175PZR
CSB-BP407175PZR
CSB-MP407175PZR
CSB-EP407175PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (mshB) CSB-YP407176MOO
CSB-EP407176MOO
CSB-BP407176MOO
CSB-MP407176MOO
CSB-EP407176MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP407177MOO
CSB-EP407177MOO
CSB-BP407177MOO
CSB-MP407177MOO
CSB-EP407177MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, partial CSB-YP407178LMH
CSB-EP407178LMH
CSB-BP407178LMH
CSB-MP407178LMH
CSB-EP407178LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton tonsurans Carboxypeptidase Y homolog A (CPYA) CSB-YP407179TQB
CSB-EP407179TQB
CSB-BP407179TQB
CSB-MP407179TQB
CSB-EP407179TQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Apocytochrome f (petA), partial CSB-YP407180GEO1
CSB-EP407180GEO1
CSB-BP407180GEO1
CSB-MP407180GEO1
CSB-EP407180GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Photosystem I reaction center subunit IX (psaJ), partial CSB-YP407181GEO
CSB-EP407181GEO
CSB-BP407181GEO
CSB-MP407181GEO
CSB-EP407181GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP407182GEO
CSB-EP407182GEO
CSB-BP407182GEO
CSB-MP407182GEO
CSB-EP407182GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Probable rRNA maturation factor (FP0002) CSB-YP407183FDU
CSB-EP407183FDU
CSB-BP407183FDU
CSB-MP407183FDU
CSB-EP407183FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Ribosome-binding factor A (rbfA) CSB-YP407184FDU
CSB-EP407184FDU
CSB-BP407184FDU
CSB-MP407184FDU
CSB-EP407184FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Aspartate--tRNA ligase (aspS), partial CSB-YP407185FDU
CSB-EP407185FDU
CSB-BP407185FDU
CSB-MP407185FDU
CSB-EP407185FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Ribosome maturation factor RimM (rimM) CSB-YP407186FDU
CSB-EP407186FDU
CSB-BP407186FDU
CSB-MP407186FDU
CSB-EP407186FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Thiazole synthase (thiG) CSB-YP407187FDU
CSB-EP407187FDU
CSB-BP407187FDU
CSB-MP407187FDU
CSB-EP407187FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum 30S ribosomal protein S2 (rpsB) CSB-YP407188FDU
CSB-EP407188FDU
CSB-BP407188FDU
CSB-MP407188FDU
CSB-EP407188FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Anthranilate phosphoribosyltransferase (trpD) CSB-YP407189FDU
CSB-EP407189FDU
CSB-BP407189FDU
CSB-MP407189FDU
CSB-EP407189FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Chaperone protein DnaK (dnaK), partial CSB-YP407190FDU
CSB-EP407190FDU
CSB-BP407190FDU
CSB-MP407190FDU
CSB-EP407190FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Histidinol-phosphate aminotransferase (hisC) CSB-YP407191FDU
CSB-EP407191FDU
CSB-BP407191FDU
CSB-MP407191FDU
CSB-EP407191FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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