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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus vannielii Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP407772MNS
CSB-EP407772MNS
CSB-BP407772MNS
CSB-MP407772MNS
CSB-EP407772MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Ribonuclease P protein component 4 (rnp4) CSB-YP407773MNS
CSB-EP407773MNS
CSB-BP407773MNS
CSB-MP407773MNS
CSB-EP407773MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Tryptophan synthase alpha chain (trpA) CSB-YP407774MNS
CSB-EP407774MNS
CSB-BP407774MNS
CSB-MP407774MNS
CSB-EP407774MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Peptide chain release factor subunit 1 (prf1) CSB-YP407775MNS
CSB-EP407775MNS
CSB-BP407775MNS
CSB-MP407775MNS
CSB-EP407775MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Hydroxyethylthiazole kinase (thiM) CSB-YP407776MNS
CSB-EP407776MNS
CSB-BP407776MNS
CSB-MP407776MNS
CSB-EP407776MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Histidinol-phosphate aminotransferase (hisC) CSB-YP407777MNS
CSB-EP407777MNS
CSB-BP407777MNS
CSB-MP407777MNS
CSB-EP407777MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 30S ribosomal protein S4P (rps4p) CSB-YP407778MNS
CSB-EP407778MNS
CSB-BP407778MNS
CSB-MP407778MNS
CSB-EP407778MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L14P (rpl14p) CSB-YP407779MNS
CSB-EP407779MNS
CSB-BP407779MNS
CSB-MP407779MNS
CSB-EP407779MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L18P (rpl18p) CSB-YP407780MNS
CSB-EP407780MNS
CSB-BP407780MNS
CSB-MP407780MNS
CSB-EP407780MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Prefoldin subunit alpha (pfdA) CSB-YP407781MNS
CSB-EP407781MNS
CSB-BP407781MNS
CSB-MP407781MNS
CSB-EP407781MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UPF0212 protein Mevan_0819 (Mevan_0819) CSB-YP407782MNS
CSB-EP407782MNS
CSB-BP407782MNS
CSB-MP407782MNS
CSB-EP407782MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP407783MNS
CSB-EP407783MNS
CSB-BP407783MNS
CSB-MP407783MNS
CSB-EP407783MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Aspartate--tRNA ligase (aspS) CSB-YP407784MNS
CSB-EP407784MNS
CSB-BP407784MNS
CSB-MP407784MNS
CSB-EP407784MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Ribosome biogenesis protein Nop10 (Mevan_1008) CSB-YP407785MNS
CSB-EP407785MNS
CSB-BP407785MNS
CSB-MP407785MNS
CSB-EP407785MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP407786MNS
CSB-EP407786MNS
CSB-BP407786MNS
CSB-MP407786MNS
CSB-EP407786MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Glutamyl-tRNA reductase (hemA) CSB-YP407787MNS
CSB-EP407787MNS
CSB-BP407787MNS
CSB-MP407787MNS
CSB-EP407787MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii L-tyrosine decarboxylase (mfnA) CSB-YP407788MNS
CSB-EP407788MNS
CSB-BP407788MNS
CSB-MP407788MNS
CSB-EP407788MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Proteasome subunit alpha (psmA) CSB-YP407789MNS
CSB-EP407789MNS
CSB-BP407789MNS
CSB-MP407789MNS
CSB-EP407789MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Homoserine kinase (thrB) CSB-YP407790MNS
CSB-EP407790MNS
CSB-BP407790MNS
CSB-MP407790MNS
CSB-EP407790MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Phosphoribosylamine--glycine ligase (purD) CSB-YP407791MNS
CSB-EP407791MNS
CSB-BP407791MNS
CSB-MP407791MNS
CSB-EP407791MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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