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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodomonas salina 50S ribosomal protein L2, chloroplastic (rpl2) CSB-YP407372RIK
CSB-EP407372RIK
CSB-BP407372RIK
CSB-MP407372RIK
CSB-EP407372RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP407373RIK
CSB-EP407373RIK
CSB-BP407373RIK
CSB-MP407373RIK
CSB-EP407373RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodomonas salina 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP407374RIK
CSB-EP407374RIK
CSB-BP407374RIK
CSB-MP407374RIK
CSB-EP407374RIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crinia riparia Riparin-1.5 amide CSB-YP407375CXJ
CSB-EP407375CXJ
CSB-BP407375CXJ
CSB-MP407375CXJ
CSB-EP407375CXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glutaredoxin-like protein C5orf63 (C5orf63) CSB-YP407376HU
CSB-EP407376HU
CSB-BP407376HU
CSB-MP407376HU
CSB-EP407376HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative golgin subfamily A member 6-like protein 11 CSB-YP407377HU
CSB-EP407377HU
CSB-BP407377HU
CSB-MP407377HU
CSB-EP407377HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UPF0627 protein ENSP00000364708 CSB-YP407378HU
CSB-EP407378HU
CSB-BP407378HU
CSB-MP407378HU
CSB-EP407378HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative methyl-CpG-binding domain protein 3-like 4 (MBD3L4) CSB-YP407379HU
CSB-EP407379HU
CSB-BP407379HU
CSB-MP407379HU
CSB-EP407379HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Puromycin-sensitive aminopeptidase-like protein (NPEPPSL1) CSB-YP407380HU
CSB-EP407380HU
CSB-BP407380HU
CSB-MP407380HU
CSB-EP407380HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative protein FAM86C-like 2 CSB-YP407381HU
CSB-EP407381HU
CSB-BP407381HU
CSB-MP407381HU
CSB-EP407381HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proline-rich protein 23A (PRR23A) CSB-YP407382HU
CSB-EP407382HU
CSB-BP407382HU
CSB-MP407382HU
CSB-EP407382HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coiled-coil domain-containing protein ENSP00000299415 CSB-YP407383HU
CSB-EP407383HU
CSB-BP407383HU
CSB-MP407383HU
CSB-EP407383HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein ENSP00000340100, partial CSB-YP407384HU
CSB-EP407384HU
CSB-BP407384HU
CSB-MP407384HU
CSB-EP407384HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA binding protein fox-1 homolog 3 (RBFOX3) CSB-YP407385HU
CSB-EP407385HU
CSB-BP407385HU
CSB-MP407385HU
CSB-EP407385HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative RNA polymerase II subunit A C-terminal domain phosphatase SSU72-like protein 3 CSB-YP407386HU
CSB-EP407386HU
CSB-BP407386HU
CSB-MP407386HU
CSB-EP407386HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein CXorf68 (CXorf68), partial CSB-YP407387HU
CSB-EP407387HU
CSB-BP407387HU
CSB-MP407387HU
CSB-EP407387HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TBC1 domain family member 3-like protein LOC729837 CSB-YP407388HU
CSB-EP407388HU
CSB-BP407388HU
CSB-MP407388HU
CSB-EP407388HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Otolin-1 (OTOL1) CSB-YP407389HU
CSB-EP407389HU
CSB-BP407389HU
CSB-MP407389HU
CSB-EP407389HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human rRNA/tRNA 2'-O-methyltransferase fibrillarin-like protein 1 (FBLL1) CSB-YP407390HU
CSB-EP407390HU
CSB-BP407390HU
CSB-MP407390HU
CSB-EP407390HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 8C (TMEM8C), partial CSB-YP407391HU1
CSB-EP407391HU1
CSB-BP407391HU1
CSB-MP407391HU1
CSB-EP407391HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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