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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinomonas sp. Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP408832MNJ
CSB-EP408832MNJ
CSB-BP408832MNJ
CSB-MP408832MNJ
CSB-EP408832MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. UPF0060 membrane protein Mmwyl1_1139 (Mmwyl1_1139), partial CSB-YP408833MNJ1
CSB-EP408833MNJ1
CSB-BP408833MNJ1
CSB-MP408833MNJ1
CSB-EP408833MNJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Glycogen synthase (glgA) CSB-YP408834MNJ
CSB-EP408834MNJ
CSB-BP408834MNJ
CSB-MP408834MNJ
CSB-EP408834MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Aspartate 1-decarboxylase (panD) CSB-YP408835MNJ
CSB-EP408835MNJ
CSB-BP408835MNJ
CSB-MP408835MNJ
CSB-EP408835MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP408836MNJ
CSB-EP408836MNJ
CSB-BP408836MNJ
CSB-MP408836MNJ
CSB-EP408836MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Catalase-peroxidase (katG), partial CSB-YP408837MNJ
CSB-EP408837MNJ
CSB-BP408837MNJ
CSB-MP408837MNJ
CSB-EP408837MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Trigger factor (tig) CSB-YP408838MNJ
CSB-EP408838MNJ
CSB-BP408838MNJ
CSB-MP408838MNJ
CSB-EP408838MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Xylose isomerase (xylA) CSB-YP408839MNJ
CSB-EP408839MNJ
CSB-BP408839MNJ
CSB-MP408839MNJ
CSB-EP408839MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP408840MNJ
CSB-EP408840MNJ
CSB-BP408840MNJ
CSB-MP408840MNJ
CSB-EP408840MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Cysteine--tRNA ligase (cysS) CSB-YP408841MNJ
CSB-EP408841MNJ
CSB-BP408841MNJ
CSB-MP408841MNJ
CSB-EP408841MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP408842MNJ
CSB-EP408842MNJ
CSB-BP408842MNJ
CSB-MP408842MNJ
CSB-EP408842MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Chorismate synthase (aroC) CSB-YP408843MNJ
CSB-EP408843MNJ
CSB-BP408843MNJ
CSB-MP408843MNJ
CSB-EP408843MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Dihydroorotase (pyrC) CSB-YP408844MNJ
CSB-EP408844MNJ
CSB-BP408844MNJ
CSB-MP408844MNJ
CSB-EP408844MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. UPF0745 protein Mmwyl1_2530 (Mmwyl1_2530) CSB-YP408845MNJ
CSB-EP408845MNJ
CSB-BP408845MNJ
CSB-MP408845MNJ
CSB-EP408845MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Protein translocase subunit SecA (secA), partial CSB-YP408846MNJ
CSB-EP408846MNJ
CSB-BP408846MNJ
CSB-MP408846MNJ
CSB-EP408846MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Protein MraZ (mraZ) CSB-YP408847MNJ
CSB-EP408847MNJ
CSB-BP408847MNJ
CSB-MP408847MNJ
CSB-EP408847MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP408848MNJ
CSB-EP408848MNJ
CSB-BP408848MNJ
CSB-MP408848MNJ
CSB-EP408848MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Serine--tRNA ligase (serS) CSB-YP408849MNJ
CSB-EP408849MNJ
CSB-BP408849MNJ
CSB-MP408849MNJ
CSB-EP408849MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP408850MNJ
CSB-EP408850MNJ
CSB-BP408850MNJ
CSB-MP408850MNJ
CSB-EP408850MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP408851MNJ
CSB-EP408851MNJ
CSB-BP408851MNJ
CSB-MP408851MNJ
CSB-EP408851MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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