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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfurovum sp. 50S ribosomal protein L36 (rpmJ) CSB-YP407452SUZ
CSB-EP407452SUZ
CSB-BP407452SUZ
CSB-MP407452SUZ
CSB-EP407452SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. 30S ribosomal protein S4 (rpsD) CSB-YP407453SUZ
CSB-EP407453SUZ
CSB-BP407453SUZ
CSB-MP407453SUZ
CSB-EP407453SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Threonine--tRNA ligase (thrS), partial CSB-YP407454SUZ
CSB-EP407454SUZ
CSB-BP407454SUZ
CSB-MP407454SUZ
CSB-EP407454SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphopentomutase (deoB) CSB-YP407455FLG
CSB-EP407455FLG
CSB-BP407455FLG
CSB-MP407455FLG
CSB-EP407455FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Recombination protein RecR (recR) CSB-YP407456FLG
CSB-EP407456FLG
CSB-BP407456FLG
CSB-MP407456FLG
CSB-EP407456FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 33 kDa chaperonin (hslO) CSB-YP407457FLG
CSB-EP407457FLG
CSB-BP407457FLG
CSB-MP407457FLG
CSB-EP407457FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribosomal protein L7Ae-like (NWMN_0506) CSB-YP407458FLG
CSB-EP407458FLG
CSB-BP407458FLG
CSB-MP407458FLG
CSB-EP407458FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucosamine-6-phosphate deaminase (nagB) CSB-YP407459FLG
CSB-EP407459FLG
CSB-BP407459FLG
CSB-MP407459FLG
CSB-EP407459FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhC2 (mnhc2), partial CSB-YP407460FLG1
CSB-EP407460FLG1
CSB-BP407460FLG1
CSB-MP407460FLG1
CSB-EP407460FLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transcriptional regulatory protein NWMN_0638 (NWMN_0638) CSB-YP407461FLG
CSB-EP407461FLG
CSB-BP407461FLG
CSB-MP407461FLG
CSB-EP407461FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein nrdI (nrdI) CSB-YP407462FLG
CSB-EP407462FLG
CSB-BP407462FLG
CSB-MP407462FLG
CSB-EP407462FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative sporulation transcription regulator WhiA (whiA) CSB-YP407463FLG
CSB-EP407463FLG
CSB-BP407463FLG
CSB-MP407463FLG
CSB-EP407463FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit E1, partial CSB-YP407464FLG1
CSB-EP407464FLG1
CSB-BP407464FLG1
CSB-MP407464FLG1
CSB-EP407464FLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP407465FLG
CSB-EP407465FLG
CSB-BP407465FLG
CSB-MP407465FLG
CSB-EP407465FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP407466FLG
CSB-EP407466FLG
CSB-BP407466FLG
CSB-MP407466FLG
CSB-EP407466FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0298 protein NWMN_0985 (NWMN_0985) CSB-YP407467FLG
CSB-EP407467FLG
CSB-BP407467FLG
CSB-MP407467FLG
CSB-EP407467FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable heme-iron transport system permease protein IsdF (isdF), partial CSB-YP407468FLG
CSB-EP407468FLG
CSB-BP407468FLG
CSB-MP407468FLG
CSB-EP407468FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0747 protein NWMN_1087 (NWMN_1087), partial CSB-YP407469FLG
CSB-EP407469FLG
CSB-BP407469FLG
CSB-MP407469FLG
CSB-EP407469FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoprotein signal peptidase (lspA), partial CSB-YP407470FLG1
CSB-EP407470FLG1
CSB-BP407470FLG1
CSB-MP407470FLG1
CSB-EP407470FLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine/threonine-protein kinase PrkC (prkC), partial CSB-YP407471FLG
CSB-EP407471FLG
CSB-BP407471FLG
CSB-MP407471FLG
CSB-EP407471FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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