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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methanococcus vannielii UPF0179 protein Mevan_0979 (Mevan_0979) CSB-YP408652MNS
CSB-EP408652MNS
CSB-BP408652MNS
CSB-MP408652MNS
CSB-EP408652MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Transcription factor E (tfe) CSB-YP408653MNS
CSB-EP408653MNS
CSB-BP408653MNS
CSB-MP408653MNS
CSB-EP408653MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii PyrE-like protein (Mevan_1091) CSB-YP408654MNS
CSB-EP408654MNS
CSB-BP408654MNS
CSB-MP408654MNS
CSB-EP408654MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP408655MNS
CSB-EP408655MNS
CSB-BP408655MNS
CSB-MP408655MNS
CSB-EP408655MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Hypoxanthine/guanine phosphoribosyltransferase (hpt) CSB-YP408656MNS
CSB-EP408656MNS
CSB-BP408656MNS
CSB-MP408656MNS
CSB-EP408656MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Putative nickel-responsive regulator (Mevan_1201) CSB-YP408657MNS
CSB-EP408657MNS
CSB-BP408657MNS
CSB-MP408657MNS
CSB-EP408657MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP408658MNS
CSB-EP408658MNS
CSB-BP408658MNS
CSB-MP408658MNS
CSB-EP408658MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UPF0248 protein Mevan_1298 (Mevan_1298) CSB-YP408659MNS
CSB-EP408659MNS
CSB-BP408659MNS
CSB-MP408659MNS
CSB-EP408659MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP408660MNS
CSB-EP408660MNS
CSB-BP408660MNS
CSB-MP408660MNS
CSB-EP408660MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Putative thymidine phosphorylase (Mevan_1338), partial CSB-YP408661MNS
CSB-EP408661MNS
CSB-BP408661MNS
CSB-MP408661MNS
CSB-EP408661MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 2-phospho-L-lactate transferase (cofD) CSB-YP408662MNS
CSB-EP408662MNS
CSB-BP408662MNS
CSB-MP408662MNS
CSB-EP408662MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UPF0218 protein Mevan_1427 (Mevan_1427) CSB-YP408663MNS
CSB-EP408663MNS
CSB-BP408663MNS
CSB-MP408663MNS
CSB-EP408663MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Peptidyl-tRNA hydrolase (pth) CSB-YP408664MNS
CSB-EP408664MNS
CSB-BP408664MNS
CSB-MP408664MNS
CSB-EP408664MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Leucine--tRNA ligase (leuS), partial CSB-YP408665MNS
CSB-EP408665MNS
CSB-BP408665MNS
CSB-MP408665MNS
CSB-EP408665MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Probable L-aspartate dehydrogenase (nadX) CSB-YP408666MNS
CSB-EP408666MNS
CSB-BP408666MNS
CSB-MP408666MNS
CSB-EP408666MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Proteasome subunit beta (psmB) CSB-YP408667MNO
CSB-EP408667MNO
CSB-BP408667MNO
CSB-MP408667MNO
CSB-EP408667MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus V-type ATP synthase subunit E (atpE) CSB-YP408668MNO
CSB-EP408668MNO
CSB-BP408668MNO
CSB-MP408668MNO
CSB-EP408668MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 3-dehydroquinate dehydratase (aroD) CSB-YP408669MNO
CSB-EP408669MNO
CSB-BP408669MNO
CSB-MP408669MNO
CSB-EP408669MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP408670MNO
CSB-EP408670MNO
CSB-BP408670MNO
CSB-MP408670MNO
CSB-EP408670MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L37Ae (rpl37Ae) CSB-YP408671MNO
CSB-EP408671MNO
CSB-BP408671MNO
CSB-MP408671MNO
CSB-EP408671MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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