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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sinorhizobium medicae Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP407752STV
CSB-EP407752STV
CSB-BP407752STV
CSB-MP407752STV
CSB-EP407752STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Uridylate kinase (pyrH) CSB-YP407753STV
CSB-EP407753STV
CSB-BP407753STV
CSB-MP407753STV
CSB-EP407753STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP407754STV
CSB-EP407754STV
CSB-BP407754STV
CSB-MP407754STV
CSB-EP407754STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Bifunctional protein GlmU (glmU) CSB-YP407755STV
CSB-EP407755STV
CSB-BP407755STV
CSB-MP407755STV
CSB-EP407755STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP407756STV
CSB-EP407756STV
CSB-BP407756STV
CSB-MP407756STV
CSB-EP407756STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP407757STV
CSB-EP407757STV
CSB-BP407757STV
CSB-MP407757STV
CSB-EP407757STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP407758STV
CSB-EP407758STV
CSB-BP407758STV
CSB-MP407758STV
CSB-EP407758STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Urease accessory protein UreF (ureF) CSB-YP407759STV
CSB-EP407759STV
CSB-BP407759STV
CSB-MP407759STV
CSB-EP407759STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Urease subunit gamma (ureA) CSB-YP407760STV
CSB-EP407760STV
CSB-BP407760STV
CSB-MP407760STV
CSB-EP407760STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Protein translocase subunit SecA (secA), partial CSB-YP407761STV
CSB-EP407761STV
CSB-BP407761STV
CSB-MP407761STV
CSB-EP407761STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Phosphoglucosamine mutase (glmM) CSB-YP407762STV
CSB-EP407762STV
CSB-BP407762STV
CSB-MP407762STV
CSB-EP407762STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP407763STV
CSB-EP407763STV
CSB-BP407763STV
CSB-MP407763STV
CSB-EP407763STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Putative pterin-4-alpha-carbinolamine dehydratase (Smed_3071) CSB-YP407764STV
CSB-EP407764STV
CSB-BP407764STV
CSB-MP407764STV
CSB-EP407764STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP407765STV
CSB-EP407765STV
CSB-BP407765STV
CSB-MP407765STV
CSB-EP407765STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP407766STV
CSB-EP407766STV
CSB-BP407766STV
CSB-MP407766STV
CSB-EP407766STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP407767STV
CSB-EP407767STV
CSB-BP407767STV
CSB-MP407767STV
CSB-EP407767STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Acid tolerance regulatory protein ActR (actR) CSB-YP407768STV
CSB-EP407768STV
CSB-BP407768STV
CSB-MP407768STV
CSB-EP407768STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Phosphopentomutase (deoB) CSB-YP407769STV
CSB-EP407769STV
CSB-BP407769STV
CSB-MP407769STV
CSB-EP407769STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Ribosome maturation factor RimP (rimP) CSB-YP407770STV
CSB-EP407770STV
CSB-BP407770STV
CSB-MP407770STV
CSB-EP407770STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Elongation factor P (efp) CSB-YP407771STV
CSB-EP407771STV
CSB-BP407771STV
CSB-MP407771STV
CSB-EP407771STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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