Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus succinogenes 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP408812AUG
CSB-EP408812AUG
CSB-BP408812AUG
CSB-MP408812AUG
CSB-EP408812AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Dihydroorotate dehydrogenase (quinone) CSB-YP408813AUG
CSB-EP408813AUG
CSB-BP408813AUG
CSB-MP408813AUG
CSB-EP408813AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphoglucosamine mutase (glmM) CSB-YP408814AUG
CSB-EP408814AUG
CSB-BP408814AUG
CSB-MP408814AUG
CSB-EP408814AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Tryptophan synthase alpha chain (trpA) CSB-YP408815AUG
CSB-EP408815AUG
CSB-BP408815AUG
CSB-MP408815AUG
CSB-EP408815AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Cytidylate kinase (cmk) CSB-YP408816AUG
CSB-EP408816AUG
CSB-BP408816AUG
CSB-MP408816AUG
CSB-EP408816AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP408817AUG
CSB-EP408817AUG
CSB-BP408817AUG
CSB-MP408817AUG
CSB-EP408817AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP408818AUG
CSB-EP408818AUG
CSB-BP408818AUG
CSB-MP408818AUG
CSB-EP408818AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP408819AUG
CSB-EP408819AUG
CSB-BP408819AUG
CSB-MP408819AUG
CSB-EP408819AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Methionine--tRNA ligase (metG), partial CSB-YP408820AUG
CSB-EP408820AUG
CSB-BP408820AUG
CSB-MP408820AUG
CSB-EP408820AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP408821AUG
CSB-EP408821AUG
CSB-BP408821AUG
CSB-MP408821AUG
CSB-EP408821AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein MraZ (mraZ) CSB-YP408822AUG
CSB-EP408822AUG
CSB-BP408822AUG
CSB-MP408822AUG
CSB-EP408822AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0235 protein Asuc_1977 (Asuc_1977) CSB-YP408823AUG
CSB-EP408823AUG
CSB-BP408823AUG
CSB-MP408823AUG
CSB-EP408823AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 7-cyano-7-deazaguanine synthase (queC) CSB-YP408824AUG
CSB-EP408824AUG
CSB-BP408824AUG
CSB-MP408824AUG
CSB-EP408824AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP408825AUG
CSB-EP408825AUG
CSB-BP408825AUG
CSB-MP408825AUG
CSB-EP408825AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L34 (rpmH) CSB-YP408826AUG
CSB-EP408826AUG
CSB-BP408826AUG
CSB-MP408826AUG
CSB-EP408826AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Putative pterin-4-alpha-carbinolamine dehydratase (Mmwyl1_0423) CSB-YP408827MNJ
CSB-EP408827MNJ
CSB-BP408827MNJ
CSB-MP408827MNJ
CSB-EP408827MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Nucleoid occlusion factor SlmA (slmA) CSB-YP408828MNJ
CSB-EP408828MNJ
CSB-BP408828MNJ
CSB-MP408828MNJ
CSB-EP408828MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP408829MNJ
CSB-EP408829MNJ
CSB-BP408829MNJ
CSB-MP408829MNJ
CSB-EP408829MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Probable Fe (2+)-trafficking protein CSB-YP408830MNJ
CSB-EP408830MNJ
CSB-BP408830MNJ
CSB-MP408830MNJ
CSB-EP408830MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Phosphoglucosamine mutase (glmM) CSB-YP408831MNJ
CSB-EP408831MNJ
CSB-BP408831MNJ
CSB-MP408831MNJ
CSB-EP408831MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>