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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP408912STM
CSB-EP408912STM
CSB-BP408912STM
CSB-MP408912STM
CSB-EP408912STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA (cmo5U34)-methyltransferase CSB-YP408913STM
CSB-EP408913STM
CSB-BP408913STM
CSB-MP408913STM
CSB-EP408913STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chaperone protein hscA homolog (hscA), partial CSB-YP408914STM
CSB-EP408914STM
CSB-BP408914STM
CSB-MP408914STM
CSB-EP408914STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP408915STM
CSB-EP408915STM
CSB-BP408915STM
CSB-MP408915STM
CSB-EP408915STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Translation initiation factor IF-1 (infA) CSB-YP408916STM
CSB-EP408916STM
CSB-BP408916STM
CSB-MP408916STM
CSB-EP408916STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Nucleoid-associated protein Shew185_2527 (Shew185_2527) CSB-YP408917STM
CSB-EP408917STM
CSB-BP408917STM
CSB-MP408917STM
CSB-EP408917STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protease HtpX (htpX), partial CSB-YP408918STM1
CSB-EP408918STM1
CSB-BP408918STM1
CSB-MP408918STM1
CSB-EP408918STM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP408919STM
CSB-EP408919STM
CSB-BP408919STM
CSB-MP408919STM
CSB-EP408919STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycosyl hydrolase family 109 protein (Shew185_2813) CSB-YP408920STM
CSB-EP408920STM
CSB-BP408920STM
CSB-MP408920STM
CSB-EP408920STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP408921STM
CSB-EP408921STM
CSB-BP408921STM
CSB-MP408921STM
CSB-EP408921STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable Fe (2+)-trafficking protein CSB-YP408922STM
CSB-EP408922STM
CSB-BP408922STM
CSB-MP408922STM
CSB-EP408922STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 5'-nucleotidase surE (surE) CSB-YP408923STM
CSB-EP408923STM
CSB-BP408923STM
CSB-MP408923STM
CSB-EP408923STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Transcriptional repressor NrdR (nrdR) CSB-YP408924STM
CSB-EP408924STM
CSB-BP408924STM
CSB-MP408924STM
CSB-EP408924STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S15 (rpsO) CSB-YP408925STM
CSB-EP408925STM
CSB-BP408925STM
CSB-MP408925STM
CSB-EP408925STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP408926STM
CSB-EP408926STM
CSB-BP408926STM
CSB-MP408926STM
CSB-EP408926STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Octanoyltransferase (lipB) CSB-YP408927STM
CSB-EP408927STM
CSB-BP408927STM
CSB-MP408927STM
CSB-EP408927STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Na (+)-translocating NADH-quinone reductase subunit D, partial CSB-YP408928STM1
CSB-EP408928STM1
CSB-BP408928STM1
CSB-MP408928STM1
CSB-EP408928STM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoglycerate kinase (pgk) CSB-YP408929STM
CSB-EP408929STM
CSB-BP408929STM
CSB-MP408929STM
CSB-EP408929STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP408930STM
CSB-EP408930STM
CSB-BP408930STM
CSB-MP408930STM
CSB-EP408930STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP408931STM
CSB-EP408931STM
CSB-BP408931STM
CSB-MP408931STM
CSB-EP408931STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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