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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Protein sprT (sprT) CSB-YP410313PZL
CSB-EP410313PZL
CSB-BP410313PZL
CSB-MP410313PZL
CSB-EP410313PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP410314PZL
CSB-EP410314PZL
CSB-BP410314PZL
CSB-MP410314PZL
CSB-EP410314PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Proline--tRNA ligase (proS), partial CSB-YP410315PZL
CSB-EP410315PZL
CSB-BP410315PZL
CSB-MP410315PZL
CSB-EP410315PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Putative pterin-4-alpha-carbinolamine dehydratase (PSPA7_4645) CSB-YP410316PZL
CSB-EP410316PZL
CSB-BP410316PZL
CSB-MP410316PZL
CSB-EP410316PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Elongation factor 4 (lepA), partial CSB-YP410317PZL
CSB-EP410317PZL
CSB-BP410317PZL
CSB-MP410317PZL
CSB-EP410317PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein translocase subunit SecA (secA), partial CSB-YP410318PZL
CSB-EP410318PZL
CSB-BP410318PZL
CSB-MP410318PZL
CSB-EP410318PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoheptose isomerase (gmhA) CSB-YP410319PZL
CSB-EP410319PZL
CSB-BP410319PZL
CSB-MP410319PZL
CSB-EP410319PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0307 protein PSPA7_5087 (PSPA7_5087) CSB-YP410320PZL
CSB-EP410320PZL
CSB-BP410320PZL
CSB-MP410320PZL
CSB-EP410320PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Isoleucine--tRNA ligase (ileS), partial CSB-YP410321PZL
CSB-EP410321PZL
CSB-BP410321PZL
CSB-MP410321PZL
CSB-EP410321PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP410322PZL
CSB-EP410322PZL
CSB-BP410322PZL
CSB-MP410322PZL
CSB-EP410322PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA (uracil (54)-C (5))-methyltransferase CSB-YP410323PZL
CSB-EP410323PZL
CSB-BP410323PZL
CSB-MP410323PZL
CSB-EP410323PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S15 (rpsO) CSB-YP410324PZL
CSB-EP410324PZL
CSB-BP410324PZL
CSB-MP410324PZL
CSB-EP410324PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoglucosamine mutase (glmM) CSB-YP410325PZL
CSB-EP410325PZL
CSB-BP410325PZL
CSB-MP410325PZL
CSB-EP410325PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa SsrA-binding protein (smpB) CSB-YP410326PZL
CSB-EP410326PZL
CSB-BP410326PZL
CSB-MP410326PZL
CSB-EP410326PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urease subunit beta (ureB) CSB-YP410327PZL
CSB-EP410327PZL
CSB-BP410327PZL
CSB-MP410327PZL
CSB-EP410327PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S6 (rpsF) CSB-YP410328PZL
CSB-EP410328PZL
CSB-BP410328PZL
CSB-MP410328PZL
CSB-EP410328PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphomethylpyrimidine synthase (thiC), partial CSB-YP410329PZL
CSB-EP410329PZL
CSB-BP410329PZL
CSB-MP410329PZL
CSB-EP410329PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa ATP-dependent protease subunit HslV (hslV) CSB-YP410330PZL
CSB-EP410330PZL
CSB-BP410330PZL
CSB-MP410330PZL
CSB-EP410330PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP410331PZL
CSB-EP410331PZL
CSB-BP410331PZL
CSB-MP410331PZL
CSB-EP410331PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP410332PZL
CSB-EP410332PZL
CSB-BP410332PZL
CSB-MP410332PZL
CSB-EP410332PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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