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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis UPF0179 protein MmarC7_0952 (MmarC7_0952) CSB-YP410353MNR
CSB-EP410353MNR
CSB-BP410353MNR
CSB-MP410353MNR
CSB-EP410353MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Geranylgeranylglyceryl phosphate synthase (MmarC7_1004) CSB-YP410354MNR
CSB-EP410354MNR
CSB-BP410354MNR
CSB-MP410354MNR
CSB-EP410354MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP410355MNR
CSB-EP410355MNR
CSB-BP410355MNR
CSB-MP410355MNR
CSB-EP410355MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis PyrE-like protein (MmarC7_1077) CSB-YP410356MNR
CSB-EP410356MNR
CSB-BP410356MNR
CSB-MP410356MNR
CSB-EP410356MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP410357MNR
CSB-EP410357MNR
CSB-BP410357MNR
CSB-MP410357MNR
CSB-EP410357MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Riboflavin kinase (ribK) CSB-YP410358MNR
CSB-EP410358MNR
CSB-BP410358MNR
CSB-MP410358MNR
CSB-EP410358MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L37Ae (rpl37Ae) CSB-YP410359MNR
CSB-EP410359MNR
CSB-BP410359MNR
CSB-MP410359MNR
CSB-EP410359MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0248 protein MmarC7_1289 (MmarC7_1289) CSB-YP410360MNR
CSB-EP410360MNR
CSB-BP410360MNR
CSB-MP410360MNR
CSB-EP410360MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Uridylate kinase (pyrH) CSB-YP410361MNR
CSB-EP410361MNR
CSB-BP410361MNR
CSB-MP410361MNR
CSB-EP410361MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein (MmarC7_1414), partial CSB-YP410362MNR
CSB-EP410362MNR
CSB-BP410362MNR
CSB-MP410362MNR
CSB-EP410362MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP410363MNR
CSB-EP410363MNR
CSB-BP410363MNR
CSB-MP410363MNR
CSB-EP410363MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L34e (rpl34e) CSB-YP410364MNR
CSB-EP410364MNR
CSB-BP410364MNR
CSB-MP410364MNR
CSB-EP410364MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ketol-acid reductoisomerase (ilvC) CSB-YP410365MNR
CSB-EP410365MNR
CSB-BP410365MNR
CSB-MP410365MNR
CSB-EP410365MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Uracil phosphoribosyltransferase (upp) CSB-YP410366MNR
CSB-EP410366MNR
CSB-BP410366MNR
CSB-MP410366MNR
CSB-EP410366MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Leucine--tRNA ligase (leuS), partial CSB-YP410367MNR
CSB-EP410367MNR
CSB-BP410367MNR
CSB-MP410367MNR
CSB-EP410367MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP410368AUG
CSB-EP410368AUG
CSB-BP410368AUG
CSB-MP410368AUG
CSB-EP410368AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L7/L12 (rplL) CSB-YP410369AUG
CSB-EP410369AUG
CSB-BP410369AUG
CSB-MP410369AUG
CSB-EP410369AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Alanine--tRNA ligase (alaS), partial CSB-YP410370AUG
CSB-EP410370AUG
CSB-BP410370AUG
CSB-MP410370AUG
CSB-EP410370AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Xanthine phosphoribosyltransferase (gpt) CSB-YP410371AUG
CSB-EP410371AUG
CSB-BP410371AUG
CSB-MP410371AUG
CSB-EP410371AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Regulator of ribonuclease activity B (rraB) CSB-YP410372AUG
CSB-EP410372AUG
CSB-BP410372AUG
CSB-MP410372AUG
CSB-EP410372AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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