Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinomonas sp. Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP412054MNJ
CSB-EP412054MNJ
CSB-BP412054MNJ
CSB-MP412054MNJ
CSB-EP412054MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. UPF0234 protein Mmwyl1_2033 (Mmwyl1_2033) CSB-YP412055MNJ
CSB-EP412055MNJ
CSB-BP412055MNJ
CSB-MP412055MNJ
CSB-EP412055MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP412056MNJ
CSB-EP412056MNJ
CSB-BP412056MNJ
CSB-MP412056MNJ
CSB-EP412056MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP412057MNJ
CSB-EP412057MNJ
CSB-BP412057MNJ
CSB-MP412057MNJ
CSB-EP412057MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP412058MNJ
CSB-EP412058MNJ
CSB-BP412058MNJ
CSB-MP412058MNJ
CSB-EP412058MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L13 (rplM) CSB-YP412059MNJ
CSB-EP412059MNJ
CSB-BP412059MNJ
CSB-MP412059MNJ
CSB-EP412059MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L20 (rplT) CSB-YP412060MNJ
CSB-EP412060MNJ
CSB-BP412060MNJ
CSB-MP412060MNJ
CSB-EP412060MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP412061MNJ
CSB-EP412061MNJ
CSB-BP412061MNJ
CSB-MP412061MNJ
CSB-EP412061MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Mannonate dehydratase (uxuA) CSB-YP412062MNJ
CSB-EP412062MNJ
CSB-BP412062MNJ
CSB-MP412062MNJ
CSB-EP412062MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP412063MNJ
CSB-EP412063MNJ
CSB-BP412063MNJ
CSB-MP412063MNJ
CSB-EP412063MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 30S ribosomal protein S18 (rpsR) CSB-YP412064MNJ
CSB-EP412064MNJ
CSB-BP412064MNJ
CSB-MP412064MNJ
CSB-EP412064MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Outer-membrane lipoprotein lolB (lolB) CSB-YP412065MNJ
CSB-EP412065MNJ
CSB-BP412065MNJ
CSB-MP412065MNJ
CSB-EP412065MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Alanine racemase (alr) CSB-YP412066MNJ
CSB-EP412066MNJ
CSB-BP412066MNJ
CSB-MP412066MNJ
CSB-EP412066MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. RNA pyrophosphohydrolase (rppH) CSB-YP412067MNJ
CSB-EP412067MNJ
CSB-BP412067MNJ
CSB-MP412067MNJ
CSB-EP412067MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L31 (rpmE) CSB-YP412068MNJ
CSB-EP412068MNJ
CSB-BP412068MNJ
CSB-MP412068MNJ
CSB-EP412068MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP412069MNJ
CSB-EP412069MNJ
CSB-BP412069MNJ
CSB-MP412069MNJ
CSB-EP412069MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Glutamate 5-kinase (proB) CSB-YP412070MNJ
CSB-EP412070MNJ
CSB-BP412070MNJ
CSB-MP412070MNJ
CSB-EP412070MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP412071MNJ
CSB-EP412071MNJ
CSB-BP412071MNJ
CSB-MP412071MNJ
CSB-EP412071MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L18 (rplR) CSB-YP412072MNJ
CSB-EP412072MNJ
CSB-BP412072MNJ
CSB-MP412072MNJ
CSB-EP412072MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L14 (rplN) CSB-YP412073MNJ
CSB-EP412073MNJ
CSB-BP412073MNJ
CSB-MP412073MNJ
CSB-EP412073MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>