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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Protein LST4 (LST4), partial CSB-YP413875STA
CSB-EP413875STA
CSB-BP413875STA
CSB-MP413875STA
CSB-EP413875STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae RNA polymerase II degradation factor 1 (DEF1), partial CSB-YP413876STA
CSB-EP413876STA
CSB-BP413876STA
CSB-MP413876STA
CSB-EP413876STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial outer membrane protein SCY_3392 (SCY_3392), partial CSB-YP413877STA1
CSB-EP413877STA1
CSB-BP413877STA1
CSB-MP413877STA1
CSB-EP413877STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Suppressor of lethality of KEX2 GAS1 double null mutant protein 1 (SKG1), partial CSB-YP413878STA
CSB-EP413878STA
CSB-BP413878STA
CSB-MP413878STA
CSB-EP413878STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae RNA annealing protein YRA2 (YRA2) CSB-YP413879STA
CSB-EP413879STA
CSB-BP413879STA
CSB-MP413879STA
CSB-EP413879STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Increased recombination centers protein 19 (IRC19) CSB-YP413880STA
CSB-EP413880STA
CSB-BP413880STA
CSB-MP413880STA
CSB-EP413880STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP413881STA
CSB-EP413881STA
CSB-BP413881STA
CSB-MP413881STA
CSB-EP413881STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Genetic interactor of prohibitin 5, mitochondrial (GEP5) CSB-YP413882STA
CSB-EP413882STA
CSB-BP413882STA
CSB-MP413882STA
CSB-EP413882STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sterol 3-beta-glucosyltransferase (ATG26), partial CSB-YP413883STA
CSB-EP413883STA
CSB-BP413883STA
CSB-MP413883STA
CSB-EP413883STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translation factor GUF1, mitochondrial (GUF1), partial CSB-YP413884STA
CSB-EP413884STA
CSB-BP413884STA
CSB-MP413884STA
CSB-EP413884STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 33 (ATG33), partial CSB-YP413885STA1
CSB-EP413885STA1
CSB-BP413885STA1
CSB-MP413885STA1
CSB-EP413885STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 54S ribosomal protein L4, mitochondrial (MRPL4), partial CSB-YP413886STA
CSB-EP413886STA
CSB-BP413886STA
CSB-MP413886STA
CSB-EP413886STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent kinase YFH7 (YFH7) CSB-YP413887STA
CSB-EP413887STA
CSB-BP413887STA
CSB-MP413887STA
CSB-EP413887STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized mitochondrial carrier SCY_1795 (SCY_1795), partial CSB-YP413888STA1
CSB-EP413888STA1
CSB-BP413888STA1
CSB-MP413888STA1
CSB-EP413888STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2), partial CSB-YP413889MRG
CSB-EP413889MRG
CSB-BP413889MRG
CSB-MP413889MRG
CSB-EP413889MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative ankyrin repeat domain-containing protein 30B-like (ANKRD30BL) CSB-YP413890HU
CSB-EP413890HU
CSB-BP413890HU
CSB-MP413890HU
CSB-EP413890HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum ATP-dependent RNA helicase dbp2 (dbp2), partial CSB-YP413891STG
CSB-EP413891STG
CSB-BP413891STG
CSB-MP413891STG
CSB-EP413891STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum rRNA biogenesis protein rrp36 (rrp36) CSB-YP413892STG
CSB-EP413892STG
CSB-BP413892STG
CSB-MP413892STG
CSB-EP413892STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum 60S ribosomal subunit assembly/export protein loc1 (loc1) CSB-YP413893STG
CSB-EP413893STG
CSB-BP413893STG
CSB-MP413893STG
CSB-EP413893STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Mitochondrial outer membrane protein iml2 (iml2), partial CSB-YP413894STG1
CSB-EP413894STG1
CSB-BP413894STG1
CSB-MP413894STG1
CSB-EP413894STG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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