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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sinorhizobium medicae 50S ribosomal protein L31 (rpmE) CSB-YP412714STV
CSB-EP412714STV
CSB-BP412714STV
CSB-MP412714STV
CSB-EP412714STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Glutamate--tRNA ligase (gltX) CSB-YP412715STV
CSB-EP412715STV
CSB-BP412715STV
CSB-MP412715STV
CSB-EP412715STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP412716STV
CSB-EP412716STV
CSB-BP412716STV
CSB-MP412716STV
CSB-EP412716STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Gamma-glutamyl phosphate reductase (proA) CSB-YP412717STV
CSB-EP412717STV
CSB-BP412717STV
CSB-MP412717STV
CSB-EP412717STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae UPF0314 protein Smed_3089 (Smed_3089), partial CSB-YP412718STV1
CSB-EP412718STV1
CSB-BP412718STV1
CSB-MP412718STV1
CSB-EP412718STV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae tRNA modification GTPase MnmE (mnmE) CSB-YP412719STV
CSB-EP412719STV
CSB-BP412719STV
CSB-MP412719STV
CSB-EP412719STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP412720STV
CSB-EP412720STV
CSB-BP412720STV
CSB-MP412720STV
CSB-EP412720STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP412721STV
CSB-EP412721STV
CSB-BP412721STV
CSB-MP412721STV
CSB-EP412721STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Adenine deaminase (Smed_3344) CSB-YP412722STV
CSB-EP412722STV
CSB-BP412722STV
CSB-MP412722STV
CSB-EP412722STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Ribosome-binding factor A (rbfA) CSB-YP412723STV
CSB-EP412723STV
CSB-BP412723STV
CSB-MP412723STV
CSB-EP412723STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP412724STV
CSB-EP412724STV
CSB-BP412724STV
CSB-MP412724STV
CSB-EP412724STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae UPF0391 membrane protein Smed_4051 (Smed_4051), partial CSB-YP412725STV
CSB-EP412725STV
CSB-BP412725STV
CSB-MP412725STV
CSB-EP412725STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Catalase-peroxidase (katG), partial CSB-YP412726STV
CSB-EP412726STV
CSB-BP412726STV
CSB-MP412726STV
CSB-EP412726STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Ribonuclease Z (rnz) CSB-YP412727MNS
CSB-EP412727MNS
CSB-BP412727MNS
CSB-MP412727MNS
CSB-EP412727MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 60 kDa chaperonin (groL), partial CSB-YP412728MNS
CSB-EP412728MNS
CSB-BP412728MNS
CSB-MP412728MNS
CSB-EP412728MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Probable ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP412729MNS
CSB-EP412729MNS
CSB-BP412729MNS
CSB-MP412729MNS
CSB-EP412729MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Arginine--tRNA ligase (argS), partial CSB-YP412730MNS
CSB-EP412730MNS
CSB-BP412730MNS
CSB-MP412730MNS
CSB-EP412730MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Phosphoglucosamine mutase (glmM) CSB-YP412731MNS
CSB-EP412731MNS
CSB-BP412731MNS
CSB-MP412731MNS
CSB-EP412731MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP412732MNS
CSB-EP412732MNS
CSB-BP412732MNS
CSB-MP412732MNS
CSB-EP412732MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP412733MNS
CSB-EP412733MNS
CSB-BP412733MNS
CSB-MP412733MNS
CSB-EP412733MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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