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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphomethylpyrimidine synthase (thiC), partial CSB-YP412593KAX
CSB-EP412593KAX
CSB-BP412593KAX
CSB-MP412593KAX
CSB-EP412593KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP412594KAX
CSB-EP412594KAX
CSB-BP412594KAX
CSB-MP412594KAX
CSB-EP412594KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Protein hfq (hfq) CSB-YP412595KAX
CSB-EP412595KAX
CSB-BP412595KAX
CSB-MP412595KAX
CSB-EP412595KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0307 protein KPN78578_45570 (KPN78578_45570) CSB-YP412596KAX
CSB-EP412596KAX
CSB-BP412596KAX
CSB-MP412596KAX
CSB-EP412596KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Chromosomal replication initiator protein DnaA (dnaA) CSB-YP412597AUP
CSB-EP412597AUP
CSB-BP412597AUP
CSB-MP412597AUP
CSB-EP412597AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UPF0182 protein Amet_0022 (Amet_0022), partial CSB-YP412598AUP
CSB-EP412598AUP
CSB-BP412598AUP
CSB-MP412598AUP
CSB-EP412598AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP412599AUP
CSB-EP412599AUP
CSB-BP412599AUP
CSB-MP412599AUP
CSB-EP412599AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Bifunctional protein GlmU (glmU) CSB-YP412600AUP
CSB-EP412600AUP
CSB-BP412600AUP
CSB-MP412600AUP
CSB-EP412600AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP412601AUP
CSB-EP412601AUP
CSB-BP412601AUP
CSB-MP412601AUP
CSB-EP412601AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Homoserine kinase (thrB) CSB-YP412602AUP
CSB-EP412602AUP
CSB-BP412602AUP
CSB-MP412602AUP
CSB-EP412602AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Chorismate synthase (aroC) CSB-YP412603AUP
CSB-EP412603AUP
CSB-BP412603AUP
CSB-MP412603AUP
CSB-EP412603AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Proline--tRNA ligase (proS), partial CSB-YP412604AUP
CSB-EP412604AUP
CSB-BP412604AUP
CSB-MP412604AUP
CSB-EP412604AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP412605AUP
CSB-EP412605AUP
CSB-BP412605AUP
CSB-MP412605AUP
CSB-EP412605AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Trigger factor (tig) CSB-YP412606AUP
CSB-EP412606AUP
CSB-BP412606AUP
CSB-MP412606AUP
CSB-EP412606AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP412607AUP
CSB-EP412607AUP
CSB-BP412607AUP
CSB-MP412607AUP
CSB-EP412607AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L35 (rpmI) CSB-YP412608AUP
CSB-EP412608AUP
CSB-BP412608AUP
CSB-MP412608AUP
CSB-EP412608AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Small, acid-soluble spore protein H (sspH) CSB-YP412609AUP
CSB-EP412609AUP
CSB-BP412609AUP
CSB-MP412609AUP
CSB-EP412609AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Cell division topological specificity factor (minE) CSB-YP412610AUP
CSB-EP412610AUP
CSB-BP412610AUP
CSB-MP412610AUP
CSB-EP412610AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP412611AUP
CSB-EP412611AUP
CSB-BP412611AUP
CSB-MP412611AUP
CSB-EP412611AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Protein hfq (hfq) CSB-YP412612AUP
CSB-EP412612AUP
CSB-BP412612AUP
CSB-MP412612AUP
CSB-EP412612AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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