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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Leucine--tRNA ligase (leuS), partial CSB-YP412793PZL
CSB-EP412793PZL
CSB-BP412793PZL
CSB-MP412793PZL
CSB-EP412793PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP412794PZL
CSB-EP412794PZL
CSB-BP412794PZL
CSB-MP412794PZL
CSB-EP412794PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Co-chaperone protein hscB homolog (hscB) CSB-YP412795PZL
CSB-EP412795PZL
CSB-BP412795PZL
CSB-MP412795PZL
CSB-EP412795PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP412796PZL
CSB-EP412796PZL
CSB-BP412796PZL
CSB-MP412796PZL
CSB-EP412796PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP412797PZL
CSB-EP412797PZL
CSB-BP412797PZL
CSB-MP412797PZL
CSB-EP412797PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP412798PZL
CSB-EP412798PZL
CSB-BP412798PZL
CSB-MP412798PZL
CSB-EP412798PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP412799PZL
CSB-EP412799PZL
CSB-BP412799PZL
CSB-MP412799PZL
CSB-EP412799PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP412800PZL
CSB-EP412800PZL
CSB-BP412800PZL
CSB-MP412800PZL
CSB-EP412800PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP412801PZL
CSB-EP412801PZL
CSB-BP412801PZL
CSB-MP412801PZL
CSB-EP412801PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0270 protein PSPA7_1664 (PSPA7_1664) CSB-YP412802PZL
CSB-EP412802PZL
CSB-BP412802PZL
CSB-MP412802PZL
CSB-EP412802PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cell division topological specificity factor (minE) CSB-YP412803PZL
CSB-EP412803PZL
CSB-BP412803PZL
CSB-MP412803PZL
CSB-EP412803PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP412804PZL
CSB-EP412804PZL
CSB-BP412804PZL
CSB-MP412804PZL
CSB-EP412804PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa LexA repressor (lexA) CSB-YP412805PZL
CSB-EP412805PZL
CSB-BP412805PZL
CSB-MP412805PZL
CSB-EP412805PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphate acyltransferase (plsX) CSB-YP412806PZL
CSB-EP412806PZL
CSB-BP412806PZL
CSB-MP412806PZL
CSB-EP412806PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP412807PZL
CSB-EP412807PZL
CSB-BP412807PZL
CSB-MP412807PZL
CSB-EP412807PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa NADH-quinone oxidoreductase subunit A 1 (nuoA1), partial CSB-YP412808PZL1
CSB-EP412808PZL1
CSB-BP412808PZL1
CSB-MP412808PZL1
CSB-EP412808PZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Serine--tRNA ligase (serS) CSB-YP412809PZL
CSB-EP412809PZL
CSB-BP412809PZL
CSB-MP412809PZL
CSB-EP412809PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Coenzyme PQQ synthesis protein D (pqqD) CSB-YP412810PZL
CSB-EP412810PZL
CSB-BP412810PZL
CSB-MP412810PZL
CSB-EP412810PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Na (+)/H (+) antiporter nhaB, partial CSB-YP412811PZL
CSB-EP412811PZL
CSB-BP412811PZL
CSB-MP412811PZL
CSB-EP412811PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Putative regulator of ribonuclease activity (PSPA7_3534) CSB-YP412812PZL
CSB-EP412812PZL
CSB-BP412812PZL
CSB-MP412812PZL
CSB-EP412812PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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