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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP412573KAX
CSB-EP412573KAX
CSB-BP412573KAX
CSB-MP412573KAX
CSB-EP412573KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0213 protein KPN78578_35340 (KPN78578_35340) CSB-YP412574KAX
CSB-EP412574KAX
CSB-BP412574KAX
CSB-MP412574KAX
CSB-EP412574KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0042 nucleotide-binding protein KPN78578_35840 (KPN78578_35840) CSB-YP412575KAX
CSB-EP412575KAX
CSB-BP412575KAX
CSB-MP412575KAX
CSB-EP412575KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP412576KAX
CSB-EP412576KAX
CSB-BP412576KAX
CSB-MP412576KAX
CSB-EP412576KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S11 (rpsK) CSB-YP412577KAX
CSB-EP412577KAX
CSB-BP412577KAX
CSB-MP412577KAX
CSB-EP412577KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S17 (rpsQ) CSB-YP412578KAX
CSB-EP412578KAX
CSB-BP412578KAX
CSB-MP412578KAX
CSB-EP412578KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Protein TusB (tusB) CSB-YP412579KAX
CSB-EP412579KAX
CSB-BP412579KAX
CSB-MP412579KAX
CSB-EP412579KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Fe/S biogenesis protein NfuA (nfuA) CSB-YP412580KAX
CSB-EP412580KAX
CSB-BP412580KAX
CSB-MP412580KAX
CSB-EP412580KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP412581KAX1
CSB-EP412581KAX1
CSB-BP412581KAX1
CSB-MP412581KAX1
CSB-EP412581KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP412582KAX
CSB-EP412582KAX
CSB-BP412582KAX
CSB-MP412582KAX
CSB-EP412582KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP412583KAX
CSB-EP412583KAX
CSB-BP412583KAX
CSB-MP412583KAX
CSB-EP412583KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP412584KAX
CSB-EP412584KAX
CSB-BP412584KAX
CSB-MP412584KAX
CSB-EP412584KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae DNA ligase B (ligB), partial CSB-YP412585KAX
CSB-EP412585KAX
CSB-BP412585KAX
CSB-MP412585KAX
CSB-EP412585KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP412586KAX
CSB-EP412586KAX
CSB-BP412586KAX
CSB-MP412586KAX
CSB-EP412586KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae ATP synthase subunit c (atpE), partial CSB-YP412587KAX1
CSB-EP412587KAX1
CSB-BP412587KAX1
CSB-MP412587KAX1
CSB-EP412587KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae D-tyrosyl-tRNA (Tyr) deacylase CSB-YP412588KAX
CSB-EP412588KAX
CSB-BP412588KAX
CSB-MP412588KAX
CSB-EP412588KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Rhamnulokinase (rhaB) CSB-YP412589KAX
CSB-EP412589KAX
CSB-BP412589KAX
CSB-MP412589KAX
CSB-EP412589KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae HTH-type transcriptional repressor fabR (fabR) CSB-YP412590KAX
CSB-EP412590KAX
CSB-BP412590KAX
CSB-MP412590KAX
CSB-EP412590KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP412591KAX
CSB-EP412591KAX
CSB-BP412591KAX
CSB-MP412591KAX
CSB-EP412591KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L11 (rplK) CSB-YP412592KAX
CSB-EP412592KAX
CSB-BP412592KAX
CSB-MP412592KAX
CSB-EP412592KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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