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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Botryotinia fuckeliana Mitochondrial thiamine pyrophosphate carrier 1 (tpc1), partial CSB-YP413233BVD1
CSB-EP413233BVD1
CSB-BP413233BVD1
CSB-MP413233BVD1
CSB-EP413233BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP413234JAQ
CSB-EP413234JAQ
CSB-BP413234JAQ
CSB-MP413234JAQ
CSB-EP413234JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP413235JAQ
CSB-EP413235JAQ
CSB-BP413235JAQ
CSB-MP413235JAQ
CSB-EP413235JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. D-tyrosyl-tRNA (Tyr) deacylase CSB-YP413236JAQ
CSB-EP413236JAQ
CSB-BP413236JAQ
CSB-MP413236JAQ
CSB-EP413236JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Thiamine-phosphate synthase (thiE) CSB-YP413237JAQ
CSB-EP413237JAQ
CSB-BP413237JAQ
CSB-MP413237JAQ
CSB-EP413237JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. GTP cyclohydrolase folE2 (folE2) CSB-YP413238JAQ
CSB-EP413238JAQ
CSB-BP413238JAQ
CSB-MP413238JAQ
CSB-EP413238JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP413239JAQ
CSB-EP413239JAQ
CSB-BP413239JAQ
CSB-MP413239JAQ
CSB-EP413239JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP413240JAQ
CSB-EP413240JAQ
CSB-BP413240JAQ
CSB-MP413240JAQ
CSB-EP413240JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 50S ribosomal protein L31 type B (rpmE2) CSB-YP413241JAQ
CSB-EP413241JAQ
CSB-BP413241JAQ
CSB-MP413241JAQ
CSB-EP413241JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Bifunctional protein FolD (folD) CSB-YP413242JAQ
CSB-EP413242JAQ
CSB-BP413242JAQ
CSB-MP413242JAQ
CSB-EP413242JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Lipoprotein signal peptidase (lspA), partial CSB-YP413243JAQ1
CSB-EP413243JAQ1
CSB-BP413243JAQ1
CSB-MP413243JAQ1
CSB-EP413243JAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP413244JAQ
CSB-EP413244JAQ
CSB-BP413244JAQ
CSB-MP413244JAQ
CSB-EP413244JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Elongation factor P (efp) CSB-YP413245JAQ
CSB-EP413245JAQ
CSB-BP413245JAQ
CSB-MP413245JAQ
CSB-EP413245JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Recombination-associated protein rdgC (rdgC) CSB-YP413246JAQ
CSB-EP413246JAQ
CSB-BP413246JAQ
CSB-MP413246JAQ
CSB-EP413246JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Protease HtpX homolog (htpX), partial CSB-YP413247JAQ1
CSB-EP413247JAQ1
CSB-BP413247JAQ1
CSB-MP413247JAQ1
CSB-EP413247JAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Phosphate acyltransferase (plsX) CSB-YP413248JAQ
CSB-EP413248JAQ
CSB-BP413248JAQ
CSB-MP413248JAQ
CSB-EP413248JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. UPF0246 protein mma_1385 (mma_1385) CSB-YP413249JAQ
CSB-EP413249JAQ
CSB-BP413249JAQ
CSB-MP413249JAQ
CSB-EP413249JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP413250JAQ
CSB-EP413250JAQ
CSB-BP413250JAQ
CSB-MP413250JAQ
CSB-EP413250JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP413251JAQ
CSB-EP413251JAQ
CSB-BP413251JAQ
CSB-MP413251JAQ
CSB-EP413251JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Urease subunit beta (ureB) CSB-YP413252JAQ
CSB-EP413252JAQ
CSB-BP413252JAQ
CSB-MP413252JAQ
CSB-EP413252JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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