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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae High frequency lysogenization protein HflD homolog (hflD) CSB-YP413294KAX
CSB-EP413294KAX
CSB-BP413294KAX
CSB-MP413294KAX
CSB-EP413294KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0249 protein KPN78578_12070 (KPN78578_12070) CSB-YP413295KAX
CSB-EP413295KAX
CSB-BP413295KAX
CSB-MP413295KAX
CSB-EP413295KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Tryptophan synthase alpha chain (trpA) CSB-YP413296KAX
CSB-EP413296KAX
CSB-BP413296KAX
CSB-MP413296KAX
CSB-EP413296KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0283 membrane protein KPN78578_12740 (KPN78578_12740), partial CSB-YP413297KAX1
CSB-EP413297KAX1
CSB-BP413297KAX1
CSB-MP413297KAX1
CSB-EP413297KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0482 protein KPN78578_15540 (KPN78578_15540) CSB-YP413298KAX
CSB-EP413298KAX
CSB-BP413298KAX
CSB-MP413298KAX
CSB-EP413298KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Catalase-peroxidase (katG), partial CSB-YP413299KAX
CSB-EP413299KAX
CSB-BP413299KAX
CSB-MP413299KAX
CSB-EP413299KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP413300KAX
CSB-EP413300KAX
CSB-BP413300KAX
CSB-MP413300KAX
CSB-EP413300KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP413301KAX
CSB-EP413301KAX
CSB-BP413301KAX
CSB-MP413301KAX
CSB-EP413301KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Fatty acid metabolism regulator protein (fadR) CSB-YP413302KAX
CSB-EP413302KAX
CSB-BP413302KAX
CSB-MP413302KAX
CSB-EP413302KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP413303KAX
CSB-EP413303KAX
CSB-BP413303KAX
CSB-MP413303KAX
CSB-EP413303KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae tRNA (mo5U34)-methyltransferase CSB-YP413304KAX
CSB-EP413304KAX
CSB-BP413304KAX
CSB-MP413304KAX
CSB-EP413304KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Protein mtfA (mtfA) CSB-YP413305KAX
CSB-EP413305KAX
CSB-BP413305KAX
CSB-MP413305KAX
CSB-EP413305KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Histidine biosynthesis bifunctional protein hisB (hisB) CSB-YP413306KAX
CSB-EP413306KAX
CSB-BP413306KAX
CSB-MP413306KAX
CSB-EP413306KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP413307KAX
CSB-EP413307KAX
CSB-BP413307KAX
CSB-MP413307KAX
CSB-EP413307KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP413308KAX
CSB-EP413308KAX
CSB-BP413308KAX
CSB-MP413308KAX
CSB-EP413308KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP413309KAX
CSB-EP413309KAX
CSB-BP413309KAX
CSB-MP413309KAX
CSB-EP413309KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP413310KAX
CSB-EP413310KAX
CSB-BP413310KAX
CSB-MP413310KAX
CSB-EP413310KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP413311KAX
CSB-EP413311KAX
CSB-BP413311KAX
CSB-MP413311KAX
CSB-EP413311KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae DNA ligase (ligA), partial CSB-YP413312KAX
CSB-EP413312KAX
CSB-BP413312KAX
CSB-MP413312KAX
CSB-EP413312KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Uracil phosphoribosyltransferase (upp) CSB-YP413313KAX
CSB-EP413313KAX
CSB-BP413313KAX
CSB-MP413313KAX
CSB-EP413313KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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