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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ajellomyces capsulata Cell division cycle protein 123 (CDC123) CSB-YP413213AUN
CSB-EP413213AUN
CSB-BP413213AUN
CSB-MP413213AUN
CSB-EP413213AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Adenylosuccinate synthetase (HCAG_06743) CSB-YP413214AUN
CSB-EP413214AUN
CSB-BP413214AUN
CSB-MP413214AUN
CSB-EP413214AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP413215AUN1
CSB-EP413215AUN1
CSB-BP413215AUN1
CSB-MP413215AUN1
CSB-EP413215AUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata 3-ketoacyl-CoA reductase (HCAG_07127), partial CSB-YP413216AUN1
CSB-EP413216AUN1
CSB-BP413216AUN1
CSB-MP413216AUN1
CSB-EP413216AUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Leukotriene A-4 hydrolase homolog (HCAG_07440), partial CSB-YP413217AUN
CSB-EP413217AUN
CSB-BP413217AUN
CSB-MP413217AUN
CSB-EP413217AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Carboxypeptidase Y homolog A (CPYA) CSB-YP413218AUN
CSB-EP413218AUN
CSB-BP413218AUN
CSB-MP413218AUN
CSB-EP413218AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Translation factor GUF1, mitochondrial (GUF1), partial CSB-YP413219AUN
CSB-EP413219AUN
CSB-BP413219AUN
CSB-MP413219AUN
CSB-EP413219AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Protein pxr1 (pxr1) CSB-YP413220BVD
CSB-EP413220BVD
CSB-BP413220BVD
CSB-MP413220BVD
CSB-EP413220BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Mitochondrial distribution and morphology protein 12 (mdm12) CSB-YP413221BVD
CSB-EP413221BVD
CSB-BP413221BVD
CSB-MP413221BVD
CSB-EP413221BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent rRNA helicase spb4 (spb4), partial CSB-YP413222BVD
CSB-EP413222BVD
CSB-BP413222BVD
CSB-MP413222BVD
CSB-EP413222BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Ribosome biogenesis protein erb1 (erb1), partial CSB-YP413223BVD
CSB-EP413223BVD
CSB-BP413223BVD
CSB-MP413223BVD
CSB-EP413223BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent RNA helicase dbp7 (dbp7), partial CSB-YP413224BVD
CSB-EP413224BVD
CSB-BP413224BVD
CSB-MP413224BVD
CSB-EP413224BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Protein get1 (get1), partial CSB-YP413225BVD1
CSB-EP413225BVD1
CSB-BP413225BVD1
CSB-MP413225BVD1
CSB-EP413225BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana WD repeat-containing protein jip5 (jip5) CSB-YP413226BVD
CSB-EP413226BVD
CSB-BP413226BVD
CSB-MP413226BVD
CSB-EP413226BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Nucleolar protein 9 (NOP9), partial CSB-YP413227BVD
CSB-EP413227BVD
CSB-BP413227BVD
CSB-MP413227BVD
CSB-EP413227BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Autophagy-related protein 3 (atg3) CSB-YP413228BVD
CSB-EP413228BVD
CSB-BP413228BVD
CSB-MP413228BVD
CSB-EP413228BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Leukotriene A-4 hydrolase homolog (BC1G_09514), partial CSB-YP413229BVD
CSB-EP413229BVD
CSB-BP413229BVD
CSB-MP413229BVD
CSB-EP413229BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Plasma membrane fusion protein prm1 (prm1), partial CSB-YP413230BVD1
CSB-EP413230BVD1
CSB-BP413230BVD1
CSB-MP413230BVD1
CSB-EP413230BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Actin cytoskeleton-regulatory complex protein end3 (end3) CSB-YP413231BVD
CSB-EP413231BVD
CSB-BP413231BVD
CSB-MP413231BVD
CSB-EP413231BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Probable Xaa-Pro aminopeptidase BC1G_13431 (BC1G_13431), partial CSB-YP413232BVD
CSB-EP413232BVD
CSB-BP413232BVD
CSB-MP413232BVD
CSB-EP413232BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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