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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Protein Xni (xni) CSB-YP413734STM
CSB-EP413734STM
CSB-BP413734STM
CSB-MP413734STM
CSB-EP413734STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Homoserine O-succinyltransferase (metA) CSB-YP413735STM
CSB-EP413735STM
CSB-BP413735STM
CSB-MP413735STM
CSB-EP413735STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP413736STM
CSB-EP413736STM
CSB-BP413736STM
CSB-MP413736STM
CSB-EP413736STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein TolB (tolB) CSB-YP413737STM
CSB-EP413737STM
CSB-BP413737STM
CSB-MP413737STM
CSB-EP413737STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0260 protein Shew185_1898 (Shew185_1898) CSB-YP413738STM
CSB-EP413738STM
CSB-BP413738STM
CSB-MP413738STM
CSB-EP413738STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP413739STM1
CSB-EP413739STM1
CSB-BP413739STM1
CSB-MP413739STM1
CSB-EP413739STM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Electron transport complex protein RnfA (rnfA), partial CSB-YP413740STM1
CSB-EP413740STM1
CSB-BP413740STM1
CSB-MP413740STM1
CSB-EP413740STM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Outer-membrane lipoprotein carrier protein (lolA) CSB-YP413741STM
CSB-EP413741STM
CSB-BP413741STM
CSB-MP413741STM
CSB-EP413741STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Thiazole synthase (thiG) CSB-YP413742STM
CSB-EP413742STM
CSB-BP413742STM
CSB-MP413742STM
CSB-EP413742STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal protein S6 modification protein 1 (rimK1) CSB-YP413743STM
CSB-EP413743STM
CSB-BP413743STM
CSB-MP413743STM
CSB-EP413743STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Uridine kinase (udk) CSB-YP413744STM
CSB-EP413744STM
CSB-BP413744STM
CSB-MP413744STM
CSB-EP413744STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Putative phosphotransferase Shew185_2482 (Shew185_2482) CSB-YP413745STM
CSB-EP413745STM
CSB-BP413745STM
CSB-MP413745STM
CSB-EP413745STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Adenylate kinase (adk) CSB-YP413746STM
CSB-EP413746STM
CSB-BP413746STM
CSB-MP413746STM
CSB-EP413746STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Anthranilate phosphoribosyltransferase (trpD) CSB-YP413747STM
CSB-EP413747STM
CSB-BP413747STM
CSB-MP413747STM
CSB-EP413747STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Proline--tRNA ligase (proS), partial CSB-YP413748STM
CSB-EP413748STM
CSB-BP413748STM
CSB-MP413748STM
CSB-EP413748STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Histidine--tRNA ligase (hisS) CSB-YP413749STM
CSB-EP413749STM
CSB-BP413749STM
CSB-MP413749STM
CSB-EP413749STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0597 protein Shew185_3080 (Shew185_3080) CSB-YP413750STM
CSB-EP413750STM
CSB-BP413750STM
CSB-MP413750STM
CSB-EP413750STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphopentomutase (deoB) CSB-YP413751STM
CSB-EP413751STM
CSB-BP413751STM
CSB-MP413751STM
CSB-EP413751STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosome maturation factor RimP (rimP) CSB-YP413752STM
CSB-EP413752STM
CSB-BP413752STM
CSB-MP413752STM
CSB-EP413752STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Leucine--tRNA ligase (leuS), partial CSB-YP413753STM
CSB-EP413753STM
CSB-BP413753STM
CSB-MP413753STM
CSB-EP413753STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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