重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對(duì)地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Parvibaculum lavamentivorans Ribose-5-phosphate isomerase A (rpiA) | CSB-YP414935PYE CSB-EP414935PYE CSB-BP414935PYE CSB-MP414935PYE CSB-EP414935PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Protein-export protein secB (secB) | CSB-YP414936PYE CSB-EP414936PYE CSB-BP414936PYE CSB-MP414936PYE CSB-EP414936PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 30S ribosomal protein S16 (rpsP) | CSB-YP414937PYE CSB-EP414937PYE CSB-BP414937PYE CSB-MP414937PYE CSB-EP414937PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Probable nicotinate-nucleotide adenylyltransferase (nadD) | CSB-YP414938PYE CSB-EP414938PYE CSB-BP414938PYE CSB-MP414938PYE CSB-EP414938PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans UPF0178 protein Plav_1521 (Plav_1521) | CSB-YP414939PYE CSB-EP414939PYE CSB-BP414939PYE CSB-MP414939PYE CSB-EP414939PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L36 (rpmJ) | CSB-YP414940PYE CSB-EP414940PYE CSB-BP414940PYE CSB-MP414940PYE CSB-EP414940PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) | CSB-YP414941PYE CSB-EP414941PYE CSB-BP414941PYE CSB-MP414941PYE CSB-EP414941PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans UPF0060 membrane protein Plav_2230 (Plav_2230) | CSB-YP414942PYE CSB-EP414942PYE CSB-BP414942PYE CSB-MP414942PYE CSB-EP414942PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans D-alanine--D-alanine ligase (ddl) | CSB-YP414943PYE CSB-EP414943PYE CSB-BP414943PYE CSB-MP414943PYE CSB-EP414943PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Alanine--tRNA ligase (alaS), partial | CSB-YP414944PYE CSB-EP414944PYE CSB-BP414944PYE CSB-MP414944PYE CSB-EP414944PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans DNA-directed RNA polymerase subunit beta' (rpoC), partial | CSB-YP414945PYE CSB-EP414945PYE CSB-BP414945PYE CSB-MP414945PYE CSB-EP414945PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L3 (rplC) | CSB-YP414946PYE CSB-EP414946PYE CSB-BP414946PYE CSB-MP414946PYE CSB-EP414946PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L5 (rplE) | CSB-YP414947PYE CSB-EP414947PYE CSB-BP414947PYE CSB-MP414947PYE CSB-EP414947PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L15 (rplO) | CSB-YP414948PYE CSB-EP414948PYE CSB-BP414948PYE CSB-MP414948PYE CSB-EP414948PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L33 (rpmG) | CSB-YP414949PYE CSB-EP414949PYE CSB-BP414949PYE CSB-MP414949PYE CSB-EP414949PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Orotate phosphoribosyltransferase (pyrE) | CSB-YP414950PYE CSB-EP414950PYE CSB-BP414950PYE CSB-MP414950PYE CSB-EP414950PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Deoxyguanosinetriphosphate triphosphohydrolase-like protein (Plav_3021) | CSB-YP414951PYE CSB-EP414951PYE CSB-BP414951PYE CSB-MP414951PYE CSB-EP414951PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Bifunctional protein GlmU (glmU) | CSB-YP414952PYE CSB-EP414952PYE CSB-BP414952PYE CSB-MP414952PYE CSB-EP414952PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Indole-3-glycerol phosphate synthase (trpC) | CSB-YP414953PYE CSB-EP414953PYE CSB-BP414953PYE CSB-MP414953PYE CSB-EP414953PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans NADH-quinone oxidoreductase subunit K (nuoK), partial | CSB-YP414954PYE1 CSB-EP414954PYE1 CSB-BP414954PYE1 CSB-MP414954PYE1 CSB-EP414954PYE1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022