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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP415134VEA
CSB-EP415134VEA
CSB-BP415134VEA
CSB-MP415134VEA
CSB-EP415134VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0208 membrane protein VIBHAR_02941 (VIBHAR_02941), partial CSB-YP415135VEA1
CSB-EP415135VEA1
CSB-BP415135VEA1
CSB-MP415135VEA1
CSB-EP415135VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP415136VEA
CSB-EP415136VEA
CSB-BP415136VEA
CSB-MP415136VEA
CSB-EP415136VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 7-cyano-7-deazaguanine synthase (queC) CSB-YP415137VEA
CSB-EP415137VEA
CSB-BP415137VEA
CSB-MP415137VEA
CSB-EP415137VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Arginine repressor (argR) CSB-YP415138VEA
CSB-EP415138VEA
CSB-BP415138VEA
CSB-MP415138VEA
CSB-EP415138VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP415139VEA
CSB-EP415139VEA
CSB-BP415139VEA
CSB-MP415139VEA
CSB-EP415139VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 30S ribosomal protein S5 (rpsE) CSB-YP415140VEA
CSB-EP415140VEA
CSB-BP415140VEA
CSB-MP415140VEA
CSB-EP415140VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L2 (rplB) CSB-YP415141VEA
CSB-EP415141VEA
CSB-BP415141VEA
CSB-MP415141VEA
CSB-EP415141VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 30S ribosomal protein S9 (rpsI) CSB-YP415142VEA
CSB-EP415142VEA
CSB-BP415142VEA
CSB-MP415142VEA
CSB-EP415142VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glutamate 5-kinase (proB) CSB-YP415143VEA
CSB-EP415143VEA
CSB-BP415143VEA
CSB-MP415143VEA
CSB-EP415143VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP415144VEA
CSB-EP415144VEA
CSB-BP415144VEA
CSB-MP415144VEA
CSB-EP415144VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 10 kDa chaperonin (groS) CSB-YP415145VEA
CSB-EP415145VEA
CSB-BP415145VEA
CSB-MP415145VEA
CSB-EP415145VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Protein hfq (hfq) CSB-YP415146VEA
CSB-EP415146VEA
CSB-BP415146VEA
CSB-MP415146VEA
CSB-EP415146VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal protein L11 methyltransferase (prmA) CSB-YP415147VEA
CSB-EP415147VEA
CSB-BP415147VEA
CSB-MP415147VEA
CSB-EP415147VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Vitamin B12-binding protein (btuF) CSB-YP415148VEA
CSB-EP415148VEA
CSB-BP415148VEA
CSB-MP415148VEA
CSB-EP415148VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP415149VEA
CSB-EP415149VEA
CSB-BP415149VEA
CSB-MP415149VEA
CSB-EP415149VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribonuclease H (rnhA) CSB-YP415150VEA
CSB-EP415150VEA
CSB-BP415150VEA
CSB-MP415150VEA
CSB-EP415150VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Outer-membrane lipoprotein lolB (lolB) CSB-YP415151VEA
CSB-EP415151VEA
CSB-BP415151VEA
CSB-MP415151VEA
CSB-EP415151VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP415152VEA
CSB-EP415152VEA
CSB-BP415152VEA
CSB-MP415152VEA
CSB-EP415152VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Probable D-serine dehydratase (dsdA) CSB-YP415153VEA
CSB-EP415153VEA
CSB-BP415153VEA
CSB-MP415153VEA
CSB-EP415153VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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