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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Campylobacter hominis 7-cyano-7-deazaguanine synthase (queC) CSB-YP414274CWD
CSB-EP414274CWD
CSB-BP414274CWD
CSB-MP414274CWD
CSB-EP414274CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP414275CWD
CSB-EP414275CWD
CSB-BP414275CWD
CSB-MP414275CWD
CSB-EP414275CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Chaperone protein htpG (htpG), partial CSB-YP414276CWD
CSB-EP414276CWD
CSB-BP414276CWD
CSB-MP414276CWD
CSB-EP414276CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP414277CWD
CSB-EP414277CWD
CSB-BP414277CWD
CSB-MP414277CWD
CSB-EP414277CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis 3-dehydroquinate synthase (aroB) CSB-YP414278CWD
CSB-EP414278CWD
CSB-BP414278CWD
CSB-MP414278CWD
CSB-EP414278CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP414279CWD
CSB-EP414279CWD
CSB-BP414279CWD
CSB-MP414279CWD
CSB-EP414279CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Transcription elongation factor GreA (greA) CSB-YP414280CWD
CSB-EP414280CWD
CSB-BP414280CWD
CSB-MP414280CWD
CSB-EP414280CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Dihydrodipicolinate reductase (dapB) CSB-YP414281CWD
CSB-EP414281CWD
CSB-BP414281CWD
CSB-MP414281CWD
CSB-EP414281CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Type III pantothenate kinase (coaX) CSB-YP414282CWD
CSB-EP414282CWD
CSB-BP414282CWD
CSB-MP414282CWD
CSB-EP414282CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP414283CWD
CSB-EP414283CWD
CSB-BP414283CWD
CSB-MP414283CWD
CSB-EP414283CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP414284CWD
CSB-EP414284CWD
CSB-BP414284CWD
CSB-MP414284CWD
CSB-EP414284CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 2-phospho-L-lactate transferase (cofD) CSB-YP414285MNV
CSB-EP414285MNV
CSB-BP414285MNV
CSB-MP414285MNV
CSB-EP414285MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Potassium-transporting ATPase A chain (kdpA), partial CSB-YP414286MNV
CSB-EP414286MNV
CSB-BP414286MNV
CSB-MP414286MNV
CSB-EP414286MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 50S ribosomal protein L3P (rpl3p) CSB-YP414287MNV
CSB-EP414287MNV
CSB-BP414287MNV
CSB-MP414287MNV
CSB-EP414287MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 30S ribosomal protein S17P (rps17p) CSB-YP414288MNV
CSB-EP414288MNV
CSB-BP414288MNV
CSB-MP414288MNV
CSB-EP414288MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Cytidylate kinase (cmk) CSB-YP414289MNV
CSB-EP414289MNV
CSB-BP414289MNV
CSB-MP414289MNV
CSB-EP414289MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 30S ribosomal protein S10P (rps10p) CSB-YP414290MNV
CSB-EP414290MNV
CSB-BP414290MNV
CSB-MP414290MNV
CSB-EP414290MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei UPF0173 metal-dependent hydrolase Mboo_0816 (Mboo_0816) CSB-YP414291MNV
CSB-EP414291MNV
CSB-BP414291MNV
CSB-MP414291MNV
CSB-EP414291MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Probable deoxycytidine triphosphate deaminase (dcd) CSB-YP414292MNV
CSB-EP414292MNV
CSB-BP414292MNV
CSB-MP414292MNV
CSB-EP414292MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei S-adenosylmethionine synthase (mat) CSB-YP414293MNV
CSB-EP414293MNV
CSB-BP414293MNV
CSB-MP414293MNV
CSB-EP414293MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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