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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Agrobacterium tumefaciens Mannosylfructose-phosphate synthase (mfpsA) CSB-YP415274AYS
CSB-EP415274AYS
CSB-BP415274AYS
CSB-MP415274AYS
CSB-EP415274AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeocystis pouchetii virus DNA polymerase (dpo), partial CSB-YP415275PYK
CSB-EP415275PYK
CSB-BP415275PYK
CSB-MP415275PYK
CSB-EP415275PYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton equinum Leucine aminopeptidase 1 (LAP1) CSB-YP415276TPZ
CSB-EP415276TPZ
CSB-BP415276TPZ
CSB-MP415276TPZ
CSB-EP415276TPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae tRNA (guanine-N (7)-)-methyltransferase subunit wuho CSB-YP415277BZL
CSB-EP415277BZL
CSB-BP415277BZL
CSB-MP415277BZL
CSB-EP415277BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Small serum protein 5 CSB-YP415278EZB
CSB-EP415278EZB
CSB-BP415278EZB
CSB-MP415278EZB
CSB-EP415278EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP415279YAN
CSB-EP415279YAN
CSB-BP415279YAN
CSB-MP415279YAN
CSB-EP415279YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Acetate kinase (ackA) CSB-YP415280YAN
CSB-EP415280YAN
CSB-BP415280YAN
CSB-MP415280YAN
CSB-EP415280YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP415281YAN
CSB-EP415281YAN
CSB-BP415281YAN
CSB-MP415281YAN
CSB-EP415281YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b DNA protection during starvation protein (dps) CSB-YP415282YAN
CSB-EP415282YAN
CSB-BP415282YAN
CSB-MP415282YAN
CSB-EP415282YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein dsrB (dsrB) CSB-YP415283YAN
CSB-EP415283YAN
CSB-BP415283YAN
CSB-MP415283YAN
CSB-EP415283YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP415284YAN
CSB-EP415284YAN
CSB-BP415284YAN
CSB-MP415284YAN
CSB-EP415284YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Integration host factor subunit alpha (ihfA) CSB-YP415285YAN
CSB-EP415285YAN
CSB-BP415285YAN
CSB-MP415285YAN
CSB-EP415285YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP415286YAN
CSB-EP415286YAN
CSB-BP415286YAN
CSB-MP415286YAN
CSB-EP415286YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Putative phosphotransferase YpsIP31758_1736 (YpsIP31758_1736) CSB-YP415287YAN
CSB-EP415287YAN
CSB-BP415287YAN
CSB-MP415287YAN
CSB-EP415287YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Transcriptional regulator slyA (slyA) CSB-YP415288YAN
CSB-EP415288YAN
CSB-BP415288YAN
CSB-MP415288YAN
CSB-EP415288YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP415289YAN
CSB-EP415289YAN
CSB-BP415289YAN
CSB-MP415289YAN
CSB-EP415289YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Tryptophan synthase alpha chain (trpA) CSB-YP415290YAN
CSB-EP415290YAN
CSB-BP415290YAN
CSB-MP415290YAN
CSB-EP415290YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0745 protein YpsIP31758_2009 (YpsIP31758_2009) CSB-YP415291YAN
CSB-EP415291YAN
CSB-BP415291YAN
CSB-MP415291YAN
CSB-EP415291YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Aspartate--tRNA ligase (aspS), partial CSB-YP415292YAN
CSB-EP415292YAN
CSB-BP415292YAN
CSB-MP415292YAN
CSB-EP415292YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Probable phosphatase YpsIP31758_2053 (YpsIP31758_2053) CSB-YP415293YAN
CSB-EP415293YAN
CSB-BP415293YAN
CSB-MP415293YAN
CSB-EP415293YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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