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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Probable metalloreductase AIM14 (AIM14), partial CSB-YP413855STA1
CSB-EP413855STA1
CSB-BP413855STA1
CSB-MP413855STA1
CSB-EP413855STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP413856STA
CSB-EP413856STA
CSB-BP413856STA
CSB-MP413856STA
CSB-EP413856STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Assembly factor CBP4 (CBP4), partial CSB-YP413857STA
CSB-EP413857STA
CSB-BP413857STA
CSB-MP413857STA
CSB-EP413857STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Stationary phase gene 1 protein (SPG1), partial CSB-YP413858STA1
CSB-EP413858STA1
CSB-BP413858STA1
CSB-MP413858STA1
CSB-EP413858STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U1 small nuclear ribonucleoprotein component SNU71 (SNU71), partial CSB-YP413859STA
CSB-EP413859STA
CSB-BP413859STA
CSB-MP413859STA
CSB-EP413859STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of rDNA transcription 14 (RRT14) CSB-YP413860STA
CSB-EP413860STA
CSB-BP413860STA
CSB-MP413860STA
CSB-EP413860STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP413861STA
CSB-EP413861STA
CSB-BP413861STA
CSB-MP413861STA
CSB-EP413861STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase MPH1 (MPH1), partial CSB-YP413862STA
CSB-EP413862STA
CSB-BP413862STA
CSB-MP413862STA
CSB-EP413862STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole component BBP1 (BBP1) CSB-YP413863STA
CSB-EP413863STA
CSB-BP413863STA
CSB-MP413863STA
CSB-EP413863STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole component 29 (SPC29) CSB-YP413864STA
CSB-EP413864STA
CSB-BP413864STA
CSB-MP413864STA
CSB-EP413864STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SAGA-associated factor 11 (SGF11) CSB-YP413865STA
CSB-EP413865STA
CSB-BP413865STA
CSB-MP413865STA
CSB-EP413865STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 8, mitochondrial (RRG8) CSB-YP413866STA
CSB-EP413866STA
CSB-BP413866STA
CSB-MP413866STA
CSB-EP413866STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA (guanine-N (7)-)-methyltransferase CSB-YP413867STA
CSB-EP413867STA
CSB-BP413867STA
CSB-MP413867STA
CSB-EP413867STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 20 (ATG20), partial CSB-YP413868STA
CSB-EP413868STA
CSB-BP413868STA
CSB-MP413868STA
CSB-EP413868STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pheromone-regulated protein PRM7 (PRM7), partial CSB-YP413869STA
CSB-EP413869STA
CSB-BP413869STA
CSB-MP413869STA
CSB-EP413869STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA (guanine-N (7)-)-methyltransferase subunit TRM82 CSB-YP413870STA
CSB-EP413870STA
CSB-BP413870STA
CSB-MP413870STA
CSB-EP413870STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nascent polypeptide-associated complex subunit beta-2 (BTT1) CSB-YP413871STA
CSB-EP413871STA
CSB-BP413871STA
CSB-MP413871STA
CSB-EP413871STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole body component 110 (SPC110), partial CSB-YP413872STA
CSB-EP413872STA
CSB-BP413872STA
CSB-MP413872STA
CSB-EP413872STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sensitive to high expression protein 9, mitochondrial (SHE9), partial CSB-YP413873STA1
CSB-EP413873STA1
CSB-BP413873STA1
CSB-MP413873STA1
CSB-EP413873STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1) CSB-YP413874STA
CSB-EP413874STA
CSB-BP413874STA
CSB-MP413874STA
CSB-EP413874STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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