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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L19 (rplS) CSB-YP414314XAV
CSB-EP414314XAV
CSB-BP414314XAV
CSB-MP414314XAV
CSB-EP414314XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP414315XAV
CSB-EP414315XAV
CSB-BP414315XAV
CSB-MP414315XAV
CSB-EP414315XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L9 (rplI) CSB-YP414316XAV
CSB-EP414316XAV
CSB-BP414316XAV
CSB-MP414316XAV
CSB-EP414316XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L11 (rplK) CSB-YP414317XAV
CSB-EP414317XAV
CSB-BP414317XAV
CSB-MP414317XAV
CSB-EP414317XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP414318XAV
CSB-EP414318XAV
CSB-BP414318XAV
CSB-MP414318XAV
CSB-EP414318XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP414319XAV
CSB-EP414319XAV
CSB-BP414319XAV
CSB-MP414319XAV
CSB-EP414319XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP414320XAV
CSB-EP414320XAV
CSB-BP414320XAV
CSB-MP414320XAV
CSB-EP414320XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Urease accessory protein UreD (ureD) CSB-YP414321XAV
CSB-EP414321XAV
CSB-BP414321XAV
CSB-MP414321XAV
CSB-EP414321XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Heme A synthase (ctaA), partial CSB-YP414322XAV
CSB-EP414322XAV
CSB-BP414322XAV
CSB-MP414322XAV
CSB-EP414322XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Phosphate acyltransferase (plsX) CSB-YP414323XAV
CSB-EP414323XAV
CSB-BP414323XAV
CSB-MP414323XAV
CSB-EP414323XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S2 (rpsB) CSB-YP414324XAV
CSB-EP414324XAV
CSB-BP414324XAV
CSB-MP414324XAV
CSB-EP414324XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP414325XAV
CSB-EP414325XAV
CSB-BP414325XAV
CSB-MP414325XAV
CSB-EP414325XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L17 (rplQ) CSB-YP414326XAV
CSB-EP414326XAV
CSB-BP414326XAV
CSB-MP414326XAV
CSB-EP414326XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L18 (rplR) CSB-YP414327XAV
CSB-EP414327XAV
CSB-BP414327XAV
CSB-MP414327XAV
CSB-EP414327XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mitogen-activated protein kinase kinase kinase 13-A (map3k13-a), partial CSB-YP414328XBE
CSB-EP414328XBE
CSB-BP414328XBE
CSB-MP414328XBE
CSB-EP414328XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP414329RIV
CSB-MP414329RIV
CSB-EP414329RIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Protein S100-A15A (S100A15A) CSB-YP414330PYX
CSB-EP414330PYX
CSB-BP414330PYX
CSB-MP414330PYX
CSB-EP414330PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Serine/threonine-protein kinase ATG1 (atg1), partial CSB-YP414331ETH
CSB-EP414331ETH
CSB-BP414331ETH
CSB-MP414331ETH
CSB-EP414331ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Autophagy-related protein 9 (atg9), partial CSB-YP414332ETH
CSB-EP414332ETH
CSB-BP414332ETH
CSB-MP414332ETH
CSB-EP414332ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Autophagy-related protein 22-2 (atg22-2), partial CSB-YP414333ETH
CSB-EP414333ETH
CSB-BP414333ETH
CSB-MP414333ETH
CSB-EP414333ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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