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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Roseiflexus castenholzii 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP414514RIP
CSB-EP414514RIP
CSB-BP414514RIP
CSB-MP414514RIP
CSB-EP414514RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L11 (rplK) CSB-YP414515RIP
CSB-EP414515RIP
CSB-BP414515RIP
CSB-MP414515RIP
CSB-EP414515RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Gamma-glutamyl phosphate reductase (proA) CSB-YP414516RIP
CSB-EP414516RIP
CSB-BP414516RIP
CSB-MP414516RIP
CSB-EP414516RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera CASP-like protein VIT_05s0020g01830 (VIT_05s0020g01830), partial CSB-YP414517VFQ1
CSB-EP414517VFQ1
CSB-BP414517VFQ1
CSB-MP414517VFQ1
CSB-EP414517VFQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Casparian strip membrane protein VIT_14s0108g01050 (VIT_14s0108g01050), partial CSB-YP414518VFQ1
CSB-EP414518VFQ1
CSB-BP414518VFQ1
CSB-MP414518VFQ1
CSB-EP414518VFQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera S-adenosylmethionine synthase 5 (METK5) CSB-YP414519VFQ
CSB-EP414519VFQ
CSB-BP414519VFQ
CSB-MP414519VFQ
CSB-EP414519VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera CASP-like protein GSVIVT00013434001 (GSVIVT00013434001), partial CSB-YP414520VFQ1
CSB-EP414520VFQ1
CSB-BP414520VFQ1
CSB-MP414520VFQ1
CSB-EP414520VFQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis UPF0732 protein v1g81173 (v1g81173) CSB-YP414521NGO
CSB-EP414521NGO
CSB-BP414521NGO
CSB-MP414521NGO
CSB-EP414521NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis SOSS complex subunit C homolog (v1g201834) CSB-YP414522NGO
CSB-EP414522NGO
CSB-BP414522NGO
CSB-MP414522NGO
CSB-EP414522NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis UPF0669 protein v1g209471 (v1g209471) CSB-YP414523NGO
CSB-EP414523NGO
CSB-BP414523NGO
CSB-MP414523NGO
CSB-EP414523NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Protein MEF2BNB homolog (v1g233178) CSB-YP414524NGO
CSB-EP414524NGO
CSB-BP414524NGO
CSB-MP414524NGO
CSB-EP414524NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Protein GDAP2 homolog (gdap2), partial CSB-YP414525NGO
CSB-EP414525NGO
CSB-BP414525NGO
CSB-MP414525NGO
CSB-EP414525NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Protein ROT1 (ROT1), partial CSB-YP414526VDW
CSB-EP414526VDW
CSB-BP414526VDW
CSB-MP414526VDW
CSB-EP414526VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora SWI5-dependent HO expression protein 2 (SHE2) CSB-YP414527VDW
CSB-EP414527VDW
CSB-BP414527VDW
CSB-MP414527VDW
CSB-EP414527VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATP-dependent RNA helicase MRH4, mitochondrial (MRH4), partial CSB-YP414528VDW
CSB-EP414528VDW
CSB-BP414528VDW
CSB-MP414528VDW
CSB-EP414528VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Pre-mRNA-splicing factor SPP381 (SPP381) CSB-YP414529VDW
CSB-EP414529VDW
CSB-BP414529VDW
CSB-MP414529VDW
CSB-EP414529VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP414530VDW
CSB-EP414530VDW
CSB-BP414530VDW
CSB-MP414530VDW
CSB-EP414530VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Autophagy-related protein 22 (ATG22), partial CSB-YP414531VDW
CSB-EP414531VDW
CSB-BP414531VDW
CSB-MP414531VDW
CSB-EP414531VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Assembly factor CBP4 (CBP4), partial CSB-YP414532VDW
CSB-EP414532VDW
CSB-BP414532VDW
CSB-MP414532VDW
CSB-EP414532VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Putative lipase ATG15 (ATG15), partial CSB-YP414533VDW
CSB-EP414533VDW
CSB-BP414533VDW
CSB-MP414533VDW
CSB-EP414533VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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