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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Morus alba Mulatexin CSB-YP414654MOE
CSB-EP414654MOE
CSB-BP414654MOE
CSB-MP414654MOE
CSB-EP414654MOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Selection and upkeep of intraepithelial T-cells protein 5 (Skint5), partial CSB-YP414655MO
CSB-EP414655MO
CSB-BP414655MO
CSB-MP414655MO
CSB-EP414655MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Chorismate synthase (aroC) CSB-YP414656YAN
CSB-EP414656YAN
CSB-BP414656YAN
CSB-MP414656YAN
CSB-EP414656YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0208 membrane protein YpsIP31758_1444 (YpsIP31758_1444), partial CSB-YP414657YAN1
CSB-EP414657YAN1
CSB-BP414657YAN1
CSB-MP414657YAN1
CSB-EP414657YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP414658YAN
CSB-EP414658YAN
CSB-BP414658YAN
CSB-MP414658YAN
CSB-EP414658YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Trans-aconitate 2-methyltransferase (tam) CSB-YP414659YAN
CSB-EP414659YAN
CSB-BP414659YAN
CSB-MP414659YAN
CSB-EP414659YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP414660YAN
CSB-EP414660YAN
CSB-BP414660YAN
CSB-MP414660YAN
CSB-EP414660YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP414661YAN
CSB-EP414661YAN
CSB-BP414661YAN
CSB-MP414661YAN
CSB-EP414661YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP414662YAN1
CSB-EP414662YAN1
CSB-BP414662YAN1
CSB-MP414662YAN1
CSB-EP414662YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b HTH-type transcriptional repressor PurR (purR) CSB-YP414663YAN
CSB-EP414663YAN
CSB-BP414663YAN
CSB-MP414663YAN
CSB-EP414663YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Tyrosine--tRNA ligase (tyrS) CSB-YP414664YAN
CSB-EP414664YAN
CSB-BP414664YAN
CSB-MP414664YAN
CSB-EP414664YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Voltage-gated ClC-type chloride channel ClcB (clcB), partial CSB-YP414665YAN
CSB-EP414665YAN
CSB-BP414665YAN
CSB-MP414665YAN
CSB-EP414665YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Electron transport complex protein RnfE (rnfE), partial CSB-YP414666YAN1
CSB-EP414666YAN1
CSB-BP414666YAN1
CSB-MP414666YAN1
CSB-EP414666YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0263 protein YpsIP31758_1954 (YpsIP31758_1954) CSB-YP414667YAN
CSB-EP414667YAN
CSB-BP414667YAN
CSB-MP414667YAN
CSB-EP414667YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Probable septum site-determining protein MinC (minC) CSB-YP414668YAN
CSB-EP414668YAN
CSB-BP414668YAN
CSB-MP414668YAN
CSB-EP414668YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP414669YAN
CSB-EP414669YAN
CSB-BP414669YAN
CSB-MP414669YAN
CSB-EP414669YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Arginine--tRNA ligase (argS), partial CSB-YP414670YAN
CSB-EP414670YAN
CSB-BP414670YAN
CSB-MP414670YAN
CSB-EP414670YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Glutamyl-tRNA reductase (hemA) CSB-YP414671YAN
CSB-EP414671YAN
CSB-BP414671YAN
CSB-MP414671YAN
CSB-EP414671YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UvrABC system protein C (uvrC), partial CSB-YP414672YAN
CSB-EP414672YAN
CSB-BP414672YAN
CSB-MP414672YAN
CSB-EP414672YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein yebF (yebF) CSB-YP414673YAN
CSB-EP414673YAN
CSB-BP414673YAN
CSB-MP414673YAN
CSB-EP414673YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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