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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum Putative 3-methyladenine DNA glycosylase (CLB_1293) CSB-YP415354CWU
CSB-EP415354CWU
CSB-BP415354CWU
CSB-MP415354CWU
CSB-EP415354CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP415355CWU
CSB-EP415355CWU
CSB-BP415355CWU
CSB-MP415355CWU
CSB-EP415355CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP415356CWU
CSB-EP415356CWU
CSB-BP415356CWU
CSB-MP415356CWU
CSB-EP415356CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA mismatch repair protein mutL (mutL), partial CSB-YP415357CWU
CSB-EP415357CWU
CSB-BP415357CWU
CSB-MP415357CWU
CSB-EP415357CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Segregation and condensation protein B (scpB) CSB-YP415358CWU
CSB-EP415358CWU
CSB-BP415358CWU
CSB-MP415358CWU
CSB-EP415358CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0597 protein CLB_1903 (CLB_1903) CSB-YP415359CWU
CSB-EP415359CWU
CSB-BP415359CWU
CSB-MP415359CWU
CSB-EP415359CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP415360CWU
CSB-EP415360CWU
CSB-BP415360CWU
CSB-MP415360CWU
CSB-EP415360CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0102 protein CLB_2304 (CLB_2304) CSB-YP415361CWU
CSB-EP415361CWU
CSB-BP415361CWU
CSB-MP415361CWU
CSB-EP415361CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0122 protein CLB_2314 (CLB_2314) CSB-YP415362CWU
CSB-EP415362CWU
CSB-BP415362CWU
CSB-MP415362CWU
CSB-EP415362CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP415363CWU
CSB-EP415363CWU
CSB-BP415363CWU
CSB-MP415363CWU
CSB-EP415363CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0297 protein CLB_2504 (CLB_2504) CSB-YP415364CWU
CSB-EP415364CWU
CSB-BP415364CWU
CSB-MP415364CWU
CSB-EP415364CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional purine biosynthesis protein PurH (purH) CSB-YP415365CWU
CSB-EP415365CWU
CSB-BP415365CWU
CSB-MP415365CWU
CSB-EP415365CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP415366CWU
CSB-EP415366CWU
CSB-BP415366CWU
CSB-MP415366CWU
CSB-EP415366CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0735 protein CLB_3034 (CLB_3034) CSB-YP415367CWU
CSB-EP415367CWU
CSB-BP415367CWU
CSB-MP415367CWU
CSB-EP415367CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP415368CWU
CSB-EP415368CWU
CSB-BP415368CWU
CSB-MP415368CWU
CSB-EP415368CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 33 kDa chaperonin (hslO) CSB-YP415369CWU
CSB-EP415369CWU
CSB-BP415369CWU
CSB-MP415369CWU
CSB-EP415369CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Orotate phosphoribosyltransferase (pyrE) CSB-YP415370CWU
CSB-EP415370CWU
CSB-BP415370CWU
CSB-MP415370CWU
CSB-EP415370CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Redox-sensing transcriptional repressor rex (rex) CSB-YP415371CWU
CSB-EP415371CWU
CSB-BP415371CWU
CSB-MP415371CWU
CSB-EP415371CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0042 nucleotide-binding protein CLB_3433 (CLB_3433) CSB-YP415372CWU
CSB-EP415372CWU
CSB-BP415372CWU
CSB-MP415372CWU
CSB-EP415372CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L13 (rplM) CSB-YP415373CWU
CSB-EP415373CWU
CSB-BP415373CWU
CSB-MP415373CWU
CSB-EP415373CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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