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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus cereus subsp. cytotoxis Hut operon positive regulatory protein (hutP) CSB-YP415434BOK
CSB-EP415434BOK
CSB-BP415434BOK
CSB-MP415434BOK
CSB-EP415434BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis LexA repressor (lexA) CSB-YP415435BOK
CSB-EP415435BOK
CSB-BP415435BOK
CSB-MP415435BOK
CSB-EP415435BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP415436BOK
CSB-EP415436BOK
CSB-BP415436BOK
CSB-MP415436BOK
CSB-EP415436BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ribosome maturation factor RimP (rimP) CSB-YP415437BOK
CSB-EP415437BOK
CSB-BP415437BOK
CSB-MP415437BOK
CSB-EP415437BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis ATP-dependent protease subunit HslV (hslV) CSB-YP415438BOK
CSB-EP415438BOK
CSB-BP415438BOK
CSB-MP415438BOK
CSB-EP415438BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0122 protein Bcer98_2498 (Bcer98_2498) CSB-YP415439BOK
CSB-EP415439BOK
CSB-BP415439BOK
CSB-MP415439BOK
CSB-EP415439BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP415440BOK
CSB-EP415440BOK
CSB-BP415440BOK
CSB-MP415440BOK
CSB-EP415440BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Bifunctional protein pyrR (pyrR) CSB-YP415441BOK
CSB-EP415441BOK
CSB-BP415441BOK
CSB-MP415441BOK
CSB-EP415441BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP415442BOK
CSB-EP415442BOK
CSB-BP415442BOK
CSB-MP415442BOK
CSB-EP415442BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Protoheme IX farnesyltransferase 2 (ctaB2), partial CSB-YP415443BOK
CSB-EP415443BOK
CSB-BP415443BOK
CSB-MP415443BOK
CSB-EP415443BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP415444BOK
CSB-EP415444BOK
CSB-BP415444BOK
CSB-MP415444BOK
CSB-EP415444BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Glucosamine-6-phosphate deaminase (nagB) CSB-YP415445BOK
CSB-EP415445BOK
CSB-BP415445BOK
CSB-MP415445BOK
CSB-EP415445BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Acetylglutamate kinase (argB) CSB-YP415446BOK
CSB-EP415446BOK
CSB-BP415446BOK
CSB-MP415446BOK
CSB-EP415446BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP415447BOK
CSB-EP415447BOK
CSB-BP415447BOK
CSB-MP415447BOK
CSB-EP415447BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP415448BOK
CSB-EP415448BOK
CSB-BP415448BOK
CSB-MP415448BOK
CSB-EP415448BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Putative phosphotransferase Bcer98_3022 (Bcer98_3022) CSB-YP415449BOK
CSB-EP415449BOK
CSB-BP415449BOK
CSB-MP415449BOK
CSB-EP415449BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Chaperone protein DnaJ (dnaJ) CSB-YP415450BOK
CSB-EP415450BOK
CSB-BP415450BOK
CSB-MP415450BOK
CSB-EP415450BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L33 2 (rpmG2) CSB-YP415451BOK
CSB-EP415451BOK
CSB-BP415451BOK
CSB-MP415451BOK
CSB-EP415451BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Putative Holliday junction resolvase (Bcer98_3099) CSB-YP415452BOK
CSB-EP415452BOK
CSB-BP415452BOK
CSB-MP415452BOK
CSB-EP415452BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP415453BOK
CSB-EP415453BOK
CSB-BP415453BOK
CSB-MP415453BOK
CSB-EP415453BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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