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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cronobacter sakazakii 50S ribosomal protein L13 (rplM) CSB-YP416414CXL
CSB-EP416414CXL
CSB-BP416414CXL
CSB-MP416414CXL
CSB-EP416414CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Flagellar protein FliT (fliT) CSB-YP416415CXL
CSB-EP416415CXL
CSB-BP416415CXL
CSB-MP416415CXL
CSB-EP416415CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Phosphoglycerate kinase (pgk) CSB-YP416416CXL
CSB-EP416416CXL
CSB-BP416416CXL
CSB-MP416416CXL
CSB-EP416416CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Undecaprenyl-diphosphatase (uppP), partial CSB-YP416417CXL
CSB-EP416417CXL
CSB-BP416417CXL
CSB-MP416417CXL
CSB-EP416417CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP416418CXL
CSB-EP416418CXL
CSB-BP416418CXL
CSB-MP416418CXL
CSB-EP416418CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Tautomerase pptA (pptA) CSB-YP416419CXL
CSB-EP416419CXL
CSB-BP416419CXL
CSB-MP416419CXL
CSB-EP416419CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Cell volume regulation protein A (cvrA), partial CSB-YP416420CXL
CSB-EP416420CXL
CSB-BP416420CXL
CSB-MP416420CXL
CSB-EP416420CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protease HtpX (htpX), partial CSB-YP416421CXL1
CSB-EP416421CXL1
CSB-BP416421CXL1
CSB-MP416421CXL1
CSB-EP416421CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0270 protein ESA_04379 (ESA_04379) CSB-YP416422CXL
CSB-EP416422CXL
CSB-BP416422CXL
CSB-MP416422CXL
CSB-EP416422CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP416423CXL
CSB-EP416423CXL
CSB-BP416423CXL
CSB-MP416423CXL
CSB-EP416423CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii GDP-mannose pyrophosphatase nudK (nudK) CSB-YP416424CXL
CSB-EP416424CXL
CSB-BP416424CXL
CSB-MP416424CXL
CSB-EP416424CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii HTH-type transcriptional activator rhaR (rhaR) CSB-YP416425CXL
CSB-EP416425CXL
CSB-BP416425CXL
CSB-MP416425CXL
CSB-EP416425CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L31 (rpmE) CSB-YP416426CXL
CSB-EP416426CXL
CSB-BP416426CXL
CSB-MP416426CXL
CSB-EP416426CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Elongation factor P-like protein (ESA_01069) CSB-YP416427CXL
CSB-EP416427CXL
CSB-BP416427CXL
CSB-MP416427CXL
CSB-EP416427CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0345 protein ESA_02910 (ESA_02910) CSB-YP416428CXL
CSB-EP416428CXL
CSB-BP416428CXL
CSB-MP416428CXL
CSB-EP416428CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP416429CXL
CSB-EP416429CXL
CSB-BP416429CXL
CSB-MP416429CXL
CSB-EP416429CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA mismatch repair protein mutL (mutL), partial CSB-YP416430CXL
CSB-EP416430CXL
CSB-BP416430CXL
CSB-MP416430CXL
CSB-EP416430CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable intracellular septation protein A (ESA_01553), partial CSB-YP416431CXL1
CSB-EP416431CXL1
CSB-BP416431CXL1
CSB-MP416431CXL1
CSB-EP416431CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Membrane-bound lysozyme inhibitor of C-type lysozyme (mliC) CSB-YP416432CXL
CSB-EP416432CXL
CSB-BP416432CXL
CSB-MP416432CXL
CSB-EP416432CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA replication terminus site-binding protein (tus) CSB-YP416433CXL
CSB-EP416433CXL
CSB-BP416433CXL
CSB-MP416433CXL
CSB-EP416433CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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