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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cronobacter sakazakii Cysteine--tRNA ligase (cysS) CSB-YP415714CXL
CSB-EP415714CXL
CSB-BP415714CXL
CSB-MP415714CXL
CSB-EP415714CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP415715CXL
CSB-EP415715CXL
CSB-BP415715CXL
CSB-MP415715CXL
CSB-EP415715CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable septum site-determining protein MinC (minC) CSB-YP415716CXL
CSB-EP415716CXL
CSB-BP415716CXL
CSB-MP415716CXL
CSB-EP415716CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein TusB (tusB) CSB-YP415717CXL
CSB-EP415717CXL
CSB-BP415717CXL
CSB-MP415717CXL
CSB-EP415717CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Universal stress protein B (uspB), partial CSB-YP415718CXL1
CSB-EP415718CXL1
CSB-BP415718CXL1
CSB-MP415718CXL1
CSB-EP415718CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA ligase (ligA), partial CSB-YP415719CXL
CSB-EP415719CXL
CSB-BP415719CXL
CSB-MP415719CXL
CSB-EP415719CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP415720CXL
CSB-EP415720CXL
CSB-BP415720CXL
CSB-MP415720CXL
CSB-EP415720CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii L-rhamnose mutarotase (rhaM) CSB-YP415721CXL
CSB-EP415721CXL
CSB-BP415721CXL
CSB-MP415721CXL
CSB-EP415721CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Acetylglutamate kinase (argB) CSB-YP415722CXL
CSB-EP415722CXL
CSB-BP415722CXL
CSB-MP415722CXL
CSB-EP415722CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP415723CXL
CSB-EP415723CXL
CSB-BP415723CXL
CSB-MP415723CXL
CSB-EP415723CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Anti-adapter protein iraP (iraP) CSB-YP415724CXL
CSB-EP415724CXL
CSB-BP415724CXL
CSB-MP415724CXL
CSB-EP415724CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0307 protein ESA_00243 (ESA_00243) CSB-YP415725CXL
CSB-EP415725CXL
CSB-BP415725CXL
CSB-MP415725CXL
CSB-EP415725CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L9 (rplI) CSB-YP415726CXL
CSB-EP415726CXL
CSB-BP415726CXL
CSB-MP415726CXL
CSB-EP415726CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 60 kDa chaperonin (groL), partial CSB-YP415727CXL
CSB-EP415727CXL
CSB-BP415727CXL
CSB-MP415727CXL
CSB-EP415727CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Tryptophan synthase alpha chain (trpA) CSB-YP415728CXL
CSB-EP415728CXL
CSB-BP415728CXL
CSB-MP415728CXL
CSB-EP415728CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii ATP synthase subunit c (atpE), partial CSB-YP415729CXL1
CSB-EP415729CXL1
CSB-BP415729CXL1
CSB-MP415729CXL1
CSB-EP415729CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ribonuclease P protein component (rnpA) CSB-YP415730CXL
CSB-EP415730CXL
CSB-BP415730CXL
CSB-MP415730CXL
CSB-EP415730CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein mgrB homolog (mgrB), partial CSB-YP415731CXL
CSB-EP415731CXL
CSB-BP415731CXL
CSB-MP415731CXL
CSB-EP415731CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Carboxylate-amine ligase ESA_02733 (ESA_02733) CSB-YP415732CXL
CSB-EP415732CXL
CSB-BP415732CXL
CSB-MP415732CXL
CSB-EP415732CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Lipid A palmitoyltransferase PagP (pagP) CSB-YP415733CXL
CSB-EP415733CXL
CSB-BP415733CXL
CSB-MP415733CXL
CSB-EP415733CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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