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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Adenylate kinase (adk) CSB-YP415754VEA
CSB-EP415754VEA
CSB-BP415754VEA
CSB-MP415754VEA
CSB-EP415754VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Trp operon repressor homolog (trpR) CSB-YP415755VEA
CSB-EP415755VEA
CSB-BP415755VEA
CSB-MP415755VEA
CSB-EP415755VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0249 protein VIBHAR_03621 (VIBHAR_03621) CSB-YP415756VEA
CSB-EP415756VEA
CSB-BP415756VEA
CSB-MP415756VEA
CSB-EP415756VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP415757VEA
CSB-EP415757VEA
CSB-BP415757VEA
CSB-MP415757VEA
CSB-EP415757VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP415758VEA
CSB-EP415758VEA
CSB-BP415758VEA
CSB-MP415758VEA
CSB-EP415758VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP415759VEA
CSB-EP415759VEA
CSB-BP415759VEA
CSB-MP415759VEA
CSB-EP415759VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP415760VEA
CSB-EP415760VEA
CSB-BP415760VEA
CSB-MP415760VEA
CSB-EP415760VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP415761VEA
CSB-EP415761VEA
CSB-BP415761VEA
CSB-MP415761VEA
CSB-EP415761VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Error-prone DNA polymerase (dnaE2), partial CSB-YP415762VEA
CSB-EP415762VEA
CSB-BP415762VEA
CSB-MP415762VEA
CSB-EP415762VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphoglucosamine mutase (glmM) CSB-YP415763VEA
CSB-EP415763VEA
CSB-BP415763VEA
CSB-MP415763VEA
CSB-EP415763VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphorelay protein luxU (luxU) CSB-YP415764VEA
CSB-EP415764VEA
CSB-BP415764VEA
CSB-MP415764VEA
CSB-EP415764VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0145 protein VIBHAR_02090 (VIBHAR_02090) CSB-YP415765VEA
CSB-EP415765VEA
CSB-BP415765VEA
CSB-MP415765VEA
CSB-EP415765VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP415766VEA
CSB-EP415766VEA
CSB-BP415766VEA
CSB-MP415766VEA
CSB-EP415766VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 30S ribosomal protein S8 (rpsH) CSB-YP415767VEA
CSB-EP415767VEA
CSB-BP415767VEA
CSB-MP415767VEA
CSB-EP415767VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L36 1 (rpmJ1) CSB-YP415768VEA
CSB-EP415768VEA
CSB-BP415768VEA
CSB-MP415768VEA
CSB-EP415768VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L22 (rplV) CSB-YP415769VEA
CSB-EP415769VEA
CSB-BP415769VEA
CSB-MP415769VEA
CSB-EP415769VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L13 (rplM) CSB-YP415770VEA
CSB-EP415770VEA
CSB-BP415770VEA
CSB-MP415770VEA
CSB-EP415770VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP415771VEA
CSB-EP415771VEA
CSB-BP415771VEA
CSB-MP415771VEA
CSB-EP415771VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP415772VEA
CSB-EP415772VEA
CSB-BP415772VEA
CSB-MP415772VEA
CSB-EP415772VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP415773VEA
CSB-EP415773VEA
CSB-BP415773VEA
CSB-MP415773VEA
CSB-EP415773VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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