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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methanoregula boonei 50S ribosomal protein L1P (rpl1p) CSB-YP415634MNV
CSB-EP415634MNV
CSB-BP415634MNV
CSB-MP415634MNV
CSB-EP415634MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei V-type ATP synthase subunit F (atpF) CSB-YP415635MNV
CSB-EP415635MNV
CSB-BP415635MNV
CSB-MP415635MNV
CSB-EP415635MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Protease HtpX homolog (htpX), partial CSB-YP415636XAV1
CSB-EP415636XAV1
CSB-BP415636XAV1
CSB-MP415636XAV1
CSB-EP415636XAV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP415637XAV
CSB-EP415637XAV
CSB-BP415637XAV
CSB-MP415637XAV
CSB-EP415637XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) CSB-YP415638XAV
CSB-EP415638XAV
CSB-BP415638XAV
CSB-MP415638XAV
CSB-EP415638XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Elongation factor 4 (lepA), partial CSB-YP415639XAV
CSB-EP415639XAV
CSB-BP415639XAV
CSB-MP415639XAV
CSB-EP415639XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP415640XAV
CSB-EP415640XAV
CSB-BP415640XAV
CSB-MP415640XAV
CSB-EP415640XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S12 (rpsL) CSB-YP415641XAV
CSB-EP415641XAV
CSB-BP415641XAV
CSB-MP415641XAV
CSB-EP415641XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S3 (rpsC) CSB-YP415642XAV
CSB-EP415642XAV
CSB-BP415642XAV
CSB-MP415642XAV
CSB-EP415642XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus DNA ligase (ligA), partial CSB-YP415643XAV
CSB-EP415643XAV
CSB-BP415643XAV
CSB-MP415643XAV
CSB-EP415643XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP415644XAV
CSB-EP415644XAV
CSB-BP415644XAV
CSB-MP415644XAV
CSB-EP415644XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP415645XAV
CSB-EP415645XAV
CSB-BP415645XAV
CSB-MP415645XAV
CSB-EP415645XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Integration host factor subunit beta (ihfB) CSB-YP415646XAV
CSB-EP415646XAV
CSB-BP415646XAV
CSB-MP415646XAV
CSB-EP415646XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Aspartate 1-decarboxylase (panD) CSB-YP415647XAV
CSB-EP415647XAV
CSB-BP415647XAV
CSB-MP415647XAV
CSB-EP415647XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribonuclease PH (rph) CSB-YP415648XAV
CSB-EP415648XAV
CSB-BP415648XAV
CSB-MP415648XAV
CSB-EP415648XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP415649XAV
CSB-EP415649XAV
CSB-BP415649XAV
CSB-MP415649XAV
CSB-EP415649XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP415650XAV
CSB-EP415650XAV
CSB-BP415650XAV
CSB-MP415650XAV
CSB-EP415650XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L10 (rplJ) CSB-YP415651XAV
CSB-EP415651XAV
CSB-BP415651XAV
CSB-MP415651XAV
CSB-EP415651XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP415652XAV
CSB-EP415652XAV
CSB-BP415652XAV
CSB-MP415652XAV
CSB-EP415652XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP415653XAV
CSB-EP415653XAV
CSB-BP415653XAV
CSB-MP415653XAV
CSB-EP415653XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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