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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anaeromyxobacter sp. UvrABC system protein C (uvrC), partial CSB-YP416234AUT
CSB-EP416234AUT
CSB-BP416234AUT
CSB-MP416234AUT
CSB-EP416234AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Translation initiation factor IF-1 (infA) CSB-YP416235AUT
CSB-EP416235AUT
CSB-BP416235AUT
CSB-MP416235AUT
CSB-EP416235AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP416236AUT
CSB-EP416236AUT
CSB-BP416236AUT
CSB-MP416236AUT
CSB-EP416236AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S20 (rpsT) CSB-YP416237AUT
CSB-EP416237AUT
CSB-BP416237AUT
CSB-MP416237AUT
CSB-EP416237AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Trigger factor (tig) CSB-YP416238AUT
CSB-EP416238AUT
CSB-BP416238AUT
CSB-MP416238AUT
CSB-EP416238AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP416239AUT
CSB-EP416239AUT
CSB-BP416239AUT
CSB-MP416239AUT
CSB-EP416239AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Na (+)/H (+) antiporter nhaA, partial CSB-YP416240AUT
CSB-EP416240AUT
CSB-BP416240AUT
CSB-MP416240AUT
CSB-EP416240AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Urease accessory protein UreD (ureD) CSB-YP416241AUT
CSB-EP416241AUT
CSB-BP416241AUT
CSB-MP416241AUT
CSB-EP416241AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP416242AUT
CSB-EP416242AUT
CSB-BP416242AUT
CSB-MP416242AUT
CSB-EP416242AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP416243AUT
CSB-EP416243AUT
CSB-BP416243AUT
CSB-MP416243AUT
CSB-EP416243AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP416244AUT
CSB-EP416244AUT
CSB-BP416244AUT
CSB-MP416244AUT
CSB-EP416244AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Putative membrane protein insertion efficiency factor (Anae109_4507) CSB-YP416245AUT
CSB-EP416245AUT
CSB-BP416245AUT
CSB-MP416245AUT
CSB-EP416245AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Lon protease (lon), partial CSB-YP416246FDS
CSB-EP416246FDS
CSB-BP416246FDS
CSB-MP416246FDS
CSB-EP416246FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Peptidyl-tRNA hydrolase (pth) CSB-YP416247FDS
CSB-EP416247FDS
CSB-BP416247FDS
CSB-MP416247FDS
CSB-EP416247FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 50S ribosomal protein L19 (rplS) CSB-YP416248FDS
CSB-EP416248FDS
CSB-BP416248FDS
CSB-MP416248FDS
CSB-EP416248FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP416249FDS
CSB-EP416249FDS
CSB-BP416249FDS
CSB-MP416249FDS
CSB-EP416249FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 50S ribosomal protein L11 (rplK) CSB-YP416250FDS
CSB-EP416250FDS
CSB-BP416250FDS
CSB-MP416250FDS
CSB-EP416250FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 5'-nucleotidase surE (surE) CSB-YP416251FDS
CSB-EP416251FDS
CSB-BP416251FDS
CSB-MP416251FDS
CSB-EP416251FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP416252FDS
CSB-EP416252FDS
CSB-BP416252FDS
CSB-MP416252FDS
CSB-EP416252FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Translation initiation factor IF-1 (infA) CSB-YP416253FDS
CSB-EP416253FDS
CSB-BP416253FDS
CSB-MP416253FDS
CSB-EP416253FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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