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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Fervidobacterium nodosum 50S ribosomal protein L6 (rplF) CSB-YP416254FDS
CSB-EP416254FDS
CSB-BP416254FDS
CSB-MP416254FDS
CSB-EP416254FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 50S ribosomal protein L16 (rplP) CSB-YP416255FDS
CSB-EP416255FDS
CSB-BP416255FDS
CSB-MP416255FDS
CSB-EP416255FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 30S ribosomal protein S19 (rpsS) CSB-YP416256FDS
CSB-EP416256FDS
CSB-BP416256FDS
CSB-MP416256FDS
CSB-EP416256FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 30S ribosomal protein S7 (rpsG) CSB-YP416257FDS
CSB-EP416257FDS
CSB-BP416257FDS
CSB-MP416257FDS
CSB-EP416257FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum L-lactate dehydrogenase (ldh) CSB-YP416258FDS
CSB-EP416258FDS
CSB-BP416258FDS
CSB-MP416258FDS
CSB-EP416258FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Cytidylate kinase (cmk) CSB-YP416259FDS
CSB-EP416259FDS
CSB-BP416259FDS
CSB-MP416259FDS
CSB-EP416259FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP416260FDS
CSB-EP416260FDS
CSB-BP416260FDS
CSB-MP416260FDS
CSB-EP416260FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Serine--tRNA ligase (serS) CSB-YP416261FDS
CSB-EP416261FDS
CSB-BP416261FDS
CSB-MP416261FDS
CSB-EP416261FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP416262FDS
CSB-EP416262FDS
CSB-BP416262FDS
CSB-MP416262FDS
CSB-EP416262FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum UPF0348 protein Fnod_1757 (Fnod_1757) CSB-YP416263FDS
CSB-EP416263FDS
CSB-BP416263FDS
CSB-MP416263FDS
CSB-EP416263FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP416264FDS
CSB-EP416264FDS
CSB-BP416264FDS
CSB-MP416264FDS
CSB-EP416264FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans UPF0391 membrane protein Plav_0056 (Plav_0056), partial CSB-YP416265PYE
CSB-EP416265PYE
CSB-BP416265PYE
CSB-MP416265PYE
CSB-EP416265PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP416266PYE
CSB-EP416266PYE
CSB-BP416266PYE
CSB-MP416266PYE
CSB-EP416266PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Dihydroorotate dehydrogenase (quinone) CSB-YP416267PYE
CSB-EP416267PYE
CSB-BP416267PYE
CSB-MP416267PYE
CSB-EP416267PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP416268PYE
CSB-EP416268PYE
CSB-BP416268PYE
CSB-MP416268PYE
CSB-EP416268PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans ATP synthase subunit b 1 (atpF1), partial CSB-YP416269PYE
CSB-EP416269PYE
CSB-BP416269PYE
CSB-MP416269PYE
CSB-EP416269PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP416270PYE
CSB-EP416270PYE
CSB-BP416270PYE
CSB-MP416270PYE
CSB-EP416270PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP416271PYE
CSB-EP416271PYE
CSB-BP416271PYE
CSB-MP416271PYE
CSB-EP416271PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans ATP-dependent protease subunit HslV (hslV) CSB-YP416272PYE
CSB-EP416272PYE
CSB-BP416272PYE
CSB-MP416272PYE
CSB-EP416272PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Leucine--tRNA ligase (leuS), partial CSB-YP416273PYE
CSB-EP416273PYE
CSB-BP416273PYE
CSB-MP416273PYE
CSB-EP416273PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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