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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Chorismate synthase (aroC) CSB-YP416454VEA
CSB-EP416454VEA
CSB-BP416454VEA
CSB-MP416454VEA
CSB-EP416454VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0307 protein VIBHAR_03672 (VIBHAR_03672) CSB-YP416455VEA
CSB-EP416455VEA
CSB-BP416455VEA
CSB-MP416455VEA
CSB-EP416455VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Putative transport protein VIBHAR_02636 (VIBHAR_02636), partial CSB-YP416456VEA
CSB-EP416456VEA
CSB-BP416456VEA
CSB-MP416456VEA
CSB-EP416456VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Serine--tRNA ligase (serS) CSB-YP416457VEA
CSB-EP416457VEA
CSB-BP416457VEA
CSB-MP416457VEA
CSB-EP416457VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Shikimate kinase (aroK) CSB-YP416458VEA
CSB-EP416458VEA
CSB-BP416458VEA
CSB-MP416458VEA
CSB-EP416458VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glutamine--tRNA ligase (glnS), partial CSB-YP416459VEA
CSB-EP416459VEA
CSB-BP416459VEA
CSB-MP416459VEA
CSB-EP416459VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Recombination-associated protein rdgC (rdgC) CSB-YP416460VEA
CSB-EP416460VEA
CSB-BP416460VEA
CSB-MP416460VEA
CSB-EP416460VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Isoleucine--tRNA ligase (ileS), partial CSB-YP416461VEA
CSB-EP416461VEA
CSB-BP416461VEA
CSB-MP416461VEA
CSB-EP416461VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP416462VEA
CSB-EP416462VEA
CSB-BP416462VEA
CSB-MP416462VEA
CSB-EP416462VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP416463VEA
CSB-EP416463VEA
CSB-BP416463VEA
CSB-MP416463VEA
CSB-EP416463VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Chaperone protein hscA homolog (hscA), partial CSB-YP416464VEA
CSB-EP416464VEA
CSB-BP416464VEA
CSB-MP416464VEA
CSB-EP416464VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP416465VEA
CSB-EP416465VEA
CSB-BP416465VEA
CSB-MP416465VEA
CSB-EP416465VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP416466VEA
CSB-EP416466VEA
CSB-BP416466VEA
CSB-MP416466VEA
CSB-EP416466VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP416467VEA
CSB-EP416467VEA
CSB-BP416467VEA
CSB-MP416467VEA
CSB-EP416467VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Integration host factor subunit alpha (ihfA) CSB-YP416468VEA
CSB-EP416468VEA
CSB-BP416468VEA
CSB-MP416468VEA
CSB-EP416468VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Imidazolonepropionase (hutI) CSB-YP416469VEA
CSB-EP416469VEA
CSB-BP416469VEA
CSB-MP416469VEA
CSB-EP416469VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L6 (rplF) CSB-YP416470VEA
CSB-EP416470VEA
CSB-BP416470VEA
CSB-MP416470VEA
CSB-EP416470VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L31 (rpmE) CSB-YP416471VEA
CSB-EP416471VEA
CSB-BP416471VEA
CSB-MP416471VEA
CSB-EP416471VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Cell division protein ZapB (zapB) CSB-YP416472VEA
CSB-EP416472VEA
CSB-BP416472VEA
CSB-MP416472VEA
CSB-EP416472VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Bifunctional protein HldE (hldE) CSB-YP416473VEA
CSB-EP416473VEA
CSB-BP416473VEA
CSB-MP416473VEA
CSB-EP416473VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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