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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus subsp. cytotoxis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP417414BOK
CSB-EP417414BOK
CSB-BP417414BOK
CSB-MP417414BOK
CSB-EP417414BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0302 protein Bcer98_1244 (Bcer98_1244) CSB-YP417415BOK
CSB-EP417415BOK
CSB-BP417415BOK
CSB-MP417415BOK
CSB-EP417415BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Xanthine phosphoribosyltransferase (xpt) CSB-YP417416BOK
CSB-EP417416BOK
CSB-BP417416BOK
CSB-MP417416BOK
CSB-EP417416BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0176 protein Bcer98_1464 (Bcer98_1464) CSB-YP417417BOK
CSB-EP417417BOK
CSB-BP417417BOK
CSB-MP417417BOK
CSB-EP417417BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis NADPH dehydrogenase (namA) CSB-YP417418BOK
CSB-EP417418BOK
CSB-BP417418BOK
CSB-MP417418BOK
CSB-EP417418BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0061 protein Bcer98_2214 (Bcer98_2214) CSB-YP417419BOK
CSB-EP417419BOK
CSB-BP417419BOK
CSB-MP417419BOK
CSB-EP417419BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Protein hfq (hfq) CSB-YP417420BOK
CSB-EP417420BOK
CSB-BP417420BOK
CSB-MP417420BOK
CSB-EP417420BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis tRNA pseudouridine synthase B (truB) CSB-YP417421BOK
CSB-EP417421BOK
CSB-BP417421BOK
CSB-MP417421BOK
CSB-EP417421BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Dihydroorotase (pyrC) CSB-YP417422BOK
CSB-EP417422BOK
CSB-BP417422BOK
CSB-MP417422BOK
CSB-EP417422BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP417423BOK
CSB-EP417423BOK
CSB-BP417423BOK
CSB-MP417423BOK
CSB-EP417423BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Heme A synthase (ctaA), partial CSB-YP417424BOK
CSB-EP417424BOK
CSB-BP417424BOK
CSB-MP417424BOK
CSB-EP417424BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Segregation and condensation protein B (scpB) CSB-YP417425BOK
CSB-EP417425BOK
CSB-BP417425BOK
CSB-MP417425BOK
CSB-EP417425BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP417426BOK
CSB-EP417426BOK
CSB-BP417426BOK
CSB-MP417426BOK
CSB-EP417426BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Transcriptional regulator MntR (mntR) CSB-YP417427BOK
CSB-EP417427BOK
CSB-BP417427BOK
CSB-MP417427BOK
CSB-EP417427BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP417428BOK
CSB-EP417428BOK
CSB-BP417428BOK
CSB-MP417428BOK
CSB-EP417428BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis DNA repair protein recO (recO) CSB-YP417429BOK
CSB-EP417429BOK
CSB-BP417429BOK
CSB-MP417429BOK
CSB-EP417429BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Protein grpE (grpE) CSB-YP417430BOK
CSB-EP417430BOK
CSB-BP417430BOK
CSB-MP417430BOK
CSB-EP417430BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Alanine--tRNA ligase (alaS), partial CSB-YP417431BOK
CSB-EP417431BOK
CSB-BP417431BOK
CSB-MP417431BOK
CSB-EP417431BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis MutS2 protein (mutS2), partial CSB-YP417432BOK
CSB-EP417432BOK
CSB-BP417432BOK
CSB-MP417432BOK
CSB-EP417432BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Transcriptional repressor NrdR (nrdR) CSB-YP417433BOK
CSB-EP417433BOK
CSB-BP417433BOK
CSB-MP417433BOK
CSB-EP417433BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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