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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthobacter autotrophicus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP417054XAV
CSB-EP417054XAV
CSB-BP417054XAV
CSB-MP417054XAV
CSB-EP417054XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP417055XAV
CSB-EP417055XAV
CSB-BP417055XAV
CSB-MP417055XAV
CSB-EP417055XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP417056XAV
CSB-EP417056XAV
CSB-BP417056XAV
CSB-MP417056XAV
CSB-EP417056XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Cobyric acid synthase (cobQ) CSB-YP417057XAV
CSB-EP417057XAV
CSB-BP417057XAV
CSB-MP417057XAV
CSB-EP417057XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Chorismate synthase (aroC) CSB-YP417058XAV
CSB-EP417058XAV
CSB-BP417058XAV
CSB-MP417058XAV
CSB-EP417058XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Putative arginyl-tRNA--protein transferase (ate) CSB-YP417059XAV
CSB-EP417059XAV
CSB-BP417059XAV
CSB-MP417059XAV
CSB-EP417059XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP417060XAV
CSB-EP417060XAV
CSB-BP417060XAV
CSB-MP417060XAV
CSB-EP417060XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP417061XAV
CSB-EP417061XAV
CSB-BP417061XAV
CSB-MP417061XAV
CSB-EP417061XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP417062XAV
CSB-EP417062XAV
CSB-BP417062XAV
CSB-MP417062XAV
CSB-EP417062XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP417063XAV
CSB-EP417063XAV
CSB-BP417063XAV
CSB-MP417063XAV
CSB-EP417063XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S13 (rpsM) CSB-YP417064XAV
CSB-EP417064XAV
CSB-BP417064XAV
CSB-MP417064XAV
CSB-EP417064XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S14 (rpsN) CSB-YP417065XAV
CSB-EP417065XAV
CSB-BP417065XAV
CSB-MP417065XAV
CSB-EP417065XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transglycosylase isaA (isaA) CSB-YP417066FLV
CSB-EP417066FLV
CSB-BP417066FLV
CSB-MP417066FLV
CSB-EP417066FLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A RNA-directed RNA polymerase, partial CSB-YP417067RIV
CSB-EP417067RIV
CSB-BP417067RIV
CSB-MP417067RIV
CSB-EP417067RIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP417068ROJ
CSB-EP417068ROJ
CSB-BP417068ROJ
CSB-MP417068ROJ
CSB-EP417068ROJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Autophagy-related protein 27 (ATG27), partial CSB-YP417069EVQ
CSB-EP417069EVQ
CSB-BP417069EVQ
CSB-MP417069EVQ
CSB-EP417069EVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Autophagy-related protein 8 (atg8) CSB-YP417070ETH
CSB-EP417070ETH
CSB-BP417070ETH
CSB-MP417070ETH
CSB-EP417070ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Autophagy-related protein 18 (atg18) CSB-YP417071ETH
CSB-EP417071ETH
CSB-BP417071ETH
CSB-MP417071ETH
CSB-EP417071ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger and SCAN domain containing protein 4D (Zscan4d) CSB-YP417072MO
CSB-EP417072MO
CSB-BP417072MO
CSB-MP417072MO
CSB-EP417072MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptelytrops albolabris Thrombin-like enzyme 2 CSB-YP417073DYI
CSB-EP417073DYI
CSB-BP417073DYI
CSB-MP417073DYI
CSB-EP417073DYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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