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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Type III pantothenate kinase (coaX) CSB-YP416774CWU
CSB-EP416774CWU
CSB-BP416774CWU
CSB-MP416774CWU
CSB-EP416774CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S18 (rpsR) CSB-YP416775CWU
CSB-EP416775CWU
CSB-BP416775CWU
CSB-MP416775CWU
CSB-EP416775CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0133 protein CLI_0048 (CLI_0048) CSB-YP416776CWW
CSB-EP416776CWW
CSB-BP416776CWW
CSB-MP416776CWW
CSB-EP416776CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase gamma chain (atpG) CSB-YP416777CWW
CSB-EP416777CWW
CSB-BP416777CWW
CSB-MP416777CWW
CSB-EP416777CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative competence-damage inducible protein (cinA) CSB-YP416778CWW
CSB-EP416778CWW
CSB-BP416778CWW
CSB-MP416778CWW
CSB-EP416778CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0371 protein CLI_0399 (CLI_0399), partial CSB-YP416779CWW
CSB-EP416779CWW
CSB-BP416779CWW
CSB-MP416779CWW
CSB-EP416779CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptidase T (pepT) CSB-YP416780CWW
CSB-EP416780CWW
CSB-BP416780CWW
CSB-MP416780CWW
CSB-EP416780CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein tlp homolog (tlp) CSB-YP416781CWW
CSB-EP416781CWW
CSB-BP416781CWW
CSB-MP416781CWW
CSB-EP416781CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Small, acid-soluble spore protein H 2 (sspH2) CSB-YP416782CWW
CSB-EP416782CWW
CSB-BP416782CWW
CSB-MP416782CWW
CSB-EP416782CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum L-lactate dehydrogenase (ldh) CSB-YP416783CWW
CSB-EP416783CWW
CSB-BP416783CWW
CSB-MP416783CWW
CSB-EP416783CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein hfq (hfq) CSB-YP416784CWW
CSB-EP416784CWW
CSB-BP416784CWW
CSB-MP416784CWW
CSB-EP416784CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Arginine repressor (argR) CSB-YP416785CWW
CSB-EP416785CWW
CSB-BP416785CWW
CSB-MP416785CWW
CSB-EP416785CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor P (efp) CSB-YP416786CWW
CSB-EP416786CWW
CSB-BP416786CWW
CSB-MP416786CWW
CSB-EP416786CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP416787CWW
CSB-EP416787CWW
CSB-BP416787CWW
CSB-MP416787CWW
CSB-EP416787CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S15 (rpsO) CSB-YP416788CWW
CSB-EP416788CWW
CSB-BP416788CWW
CSB-MP416788CWW
CSB-EP416788CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP416789CWW
CSB-EP416789CWW
CSB-BP416789CWW
CSB-MP416789CWW
CSB-EP416789CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome maturation factor RimM (rimM) CSB-YP416790CWW
CSB-EP416790CWW
CSB-BP416790CWW
CSB-MP416790CWW
CSB-EP416790CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetate kinase (ackA) CSB-YP416791CWW
CSB-EP416791CWW
CSB-BP416791CWW
CSB-MP416791CWW
CSB-EP416791CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP416792CWW
CSB-EP416792CWW
CSB-BP416792CWW
CSB-MP416792CWW
CSB-EP416792CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative Holliday junction resolvase (CLI_2625) CSB-YP416793CWW
CSB-EP416793CWW
CSB-BP416793CWW
CSB-MP416793CWW
CSB-EP416793CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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