重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L21 (rplU) | CSB-YP416974PYE CSB-EP416974PYE CSB-BP416974PYE CSB-MP416974PYE CSB-EP416974PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Probable transcriptional regulatory protein Plav_2114 (Plav_2114) | CSB-YP416975PYE CSB-EP416975PYE CSB-BP416975PYE CSB-MP416975PYE CSB-EP416975PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans UPF0234 protein Plav_2177 (Plav_2177) | CSB-YP416976PYE CSB-EP416976PYE CSB-BP416976PYE CSB-MP416976PYE CSB-EP416976PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Protein MraZ (mraZ) | CSB-YP416977PYE CSB-EP416977PYE CSB-BP416977PYE CSB-MP416977PYE CSB-EP416977PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 30S ribosomal protein S7 (rpsG) | CSB-YP416978PYE CSB-EP416978PYE CSB-BP416978PYE CSB-MP416978PYE CSB-EP416978PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L29 (rpmC) | CSB-YP416979PYE CSB-EP416979PYE CSB-BP416979PYE CSB-MP416979PYE CSB-EP416979PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L6 (rplF) | CSB-YP416980PYE CSB-EP416980PYE CSB-BP416980PYE CSB-MP416980PYE CSB-EP416980PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans DNA-directed RNA polymerase subunit alpha (rpoA) | CSB-YP416981PYE CSB-EP416981PYE CSB-BP416981PYE CSB-MP416981PYE CSB-EP416981PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans DNA-directed RNA polymerase subunit omega (rpoZ) | CSB-YP416982PYE CSB-EP416982PYE CSB-BP416982PYE CSB-MP416982PYE CSB-EP416982PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Putative arginyl-tRNA--protein transferase (ate) | CSB-YP416983PYE CSB-EP416983PYE CSB-BP416983PYE CSB-MP416983PYE CSB-EP416983PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Serine--tRNA ligase (serS) | CSB-YP416984PYE CSB-EP416984PYE CSB-BP416984PYE CSB-MP416984PYE CSB-EP416984PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Bifunctional enzyme IspD/IspF (ispDF) | CSB-YP416985PYE CSB-EP416985PYE CSB-BP416985PYE CSB-MP416985PYE CSB-EP416985PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase | CSB-YP416986PYE CSB-EP416986PYE CSB-BP416986PYE CSB-MP416986PYE CSB-EP416986PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans NADH-quinone oxidoreductase subunit N (nuoN), partial | CSB-YP416987PYE CSB-EP416987PYE CSB-BP416987PYE CSB-MP416987PYE CSB-EP416987PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Trigger factor (tig), partial | CSB-YP416988PYE CSB-EP416988PYE CSB-BP416988PYE CSB-MP416988PYE CSB-EP416988PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L9 (rplI) | CSB-YP416989PYE CSB-EP416989PYE CSB-BP416989PYE CSB-MP416989PYE CSB-EP416989PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Chaperone protein DnaK (dnaK), partial | CSB-YP416990PYE CSB-EP416990PYE CSB-BP416990PYE CSB-MP416990PYE CSB-EP416990PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Parvibaculum lavamentivorans Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) | CSB-YP416991PYE CSB-EP416991PYE CSB-BP416991PYE CSB-MP416991PYE CSB-EP416991PYE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Campylobacter hominis 6,7-dimethyl-8-ribityllumazine synthase (ribH) | CSB-YP416992CWD CSB-EP416992CWD CSB-BP416992CWD CSB-MP416992CWD CSB-EP416992CWD-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Campylobacter hominis 50S ribosomal protein L2 (rplB) | CSB-YP416993CWD CSB-EP416993CWD CSB-BP416993CWD CSB-MP416993CWD CSB-EP416993CWD-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022