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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum 50S ribosomal protein L27 (rpmA) CSB-YP417354CWU
CSB-EP417354CWU
CSB-BP417354CWU
CSB-MP417354CWU
CSB-EP417354CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate--tRNA ligase (aspS), partial CSB-YP417355CWU
CSB-EP417355CWU
CSB-BP417355CWU
CSB-MP417355CWU
CSB-EP417355CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Threonine--tRNA ligase (thrS), partial CSB-YP417356CWU
CSB-EP417356CWU
CSB-BP417356CWU
CSB-MP417356CWU
CSB-EP417356CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 60 kDa chaperonin (groL), partial CSB-YP417357CWU
CSB-EP417357CWU
CSB-BP417357CWU
CSB-MP417357CWU
CSB-EP417357CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S11 (rpsK) CSB-YP417358CWU
CSB-EP417358CWU
CSB-BP417358CWU
CSB-MP417358CWU
CSB-EP417358CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L18 (rplR) CSB-YP417359CWU
CSB-EP417359CWU
CSB-BP417359CWU
CSB-MP417359CWU
CSB-EP417359CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S17 (rpsQ) CSB-YP417360CWU
CSB-EP417360CWU
CSB-BP417360CWU
CSB-MP417360CWU
CSB-EP417360CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L3 (rplC) CSB-YP417361CWU
CSB-EP417361CWU
CSB-BP417361CWU
CSB-MP417361CWU
CSB-EP417361CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP417362CWU
CSB-EP417362CWU
CSB-BP417362CWU
CSB-MP417362CWU
CSB-EP417362CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP417363CWU
CSB-EP417363CWU
CSB-BP417363CWU
CSB-MP417363CWU
CSB-EP417363CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Adenylosuccinate synthetase (purA) CSB-YP417364CWU
CSB-EP417364CWU
CSB-BP417364CWU
CSB-MP417364CWU
CSB-EP417364CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0182 protein CLI_0022 (CLI_0022), partial CSB-YP417365CWW
CSB-EP417365CWW
CSB-BP417365CWW
CSB-MP417365CWW
CSB-EP417365CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L31 (rpmE) CSB-YP417366CWW
CSB-EP417366CWW
CSB-BP417366CWW
CSB-MP417366CWW
CSB-EP417366CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine synthase (metK) CSB-YP417367CWW
CSB-EP417367CWW
CSB-BP417367CWW
CSB-MP417367CWW
CSB-EP417367CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0059 membrane protein CLI_0469 (CLI_0469), partial CSB-YP417368CWW1
CSB-EP417368CWW1
CSB-BP417368CWW1
CSB-MP417368CWW1
CSB-EP417368CWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Thiamine-phosphate synthase (thiE) CSB-YP417369CWW
CSB-EP417369CWW
CSB-BP417369CWW
CSB-MP417369CWW
CSB-EP417369CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Arginine--tRNA ligase (argS), partial CSB-YP417370CWW
CSB-EP417370CWW
CSB-BP417370CWW
CSB-MP417370CWW
CSB-EP417370CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP417371CWW
CSB-EP417371CWW
CSB-BP417371CWW
CSB-MP417371CWW
CSB-EP417371CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP phosphoribosyltransferase (hisG) CSB-YP417372CWW
CSB-EP417372CWW
CSB-BP417372CWW
CSB-MP417372CWW
CSB-EP417372CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum LexA repressor (lexA) CSB-YP417373CWW
CSB-EP417373CWW
CSB-BP417373CWW
CSB-MP417373CWW
CSB-EP417373CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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