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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype O:1b Chaperone protein DnaJ (dnaJ) CSB-YP418714YAN
CSB-EP418714YAN
CSB-BP418714YAN
CSB-MP418714YAN
CSB-EP418714YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Non-canonical purine NTP phosphatase (YpsIP31758_3483) CSB-YP418715YAN
CSB-EP418715YAN
CSB-BP418715YAN
CSB-MP418715YAN
CSB-EP418715YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Autoinducer 2 import ATP-binding protein LsrA (lsrA), partial CSB-YP418716YAN
CSB-EP418716YAN
CSB-BP418716YAN
CSB-MP418716YAN
CSB-EP418716YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0306 protein YpsIP31758_3570 (YpsIP31758_3570) CSB-YP418717YAN
CSB-EP418717YAN
CSB-BP418717YAN
CSB-MP418717YAN
CSB-EP418717YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L21 (rplU) CSB-YP418718YAN
CSB-EP418718YAN
CSB-BP418718YAN
CSB-MP418718YAN
CSB-EP418718YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b HTH-type transcriptional repressor NsrR (nsrR) CSB-YP418719YAN
CSB-EP418719YAN
CSB-BP418719YAN
CSB-MP418719YAN
CSB-EP418719YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b HTH-type transcriptional activator rhaS (rhaS) CSB-YP418720YAN
CSB-EP418720YAN
CSB-BP418720YAN
CSB-MP418720YAN
CSB-EP418720YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Regulator of sigma D (rsd) CSB-YP418721YAN
CSB-EP418721YAN
CSB-BP418721YAN
CSB-MP418721YAN
CSB-EP418721YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L11 (rplK) CSB-YP418722YAN
CSB-EP418722YAN
CSB-BP418722YAN
CSB-MP418722YAN
CSB-EP418722YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP418723YAN
CSB-EP418723YAN
CSB-BP418723YAN
CSB-MP418723YAN
CSB-EP418723YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L30 (rpmD) CSB-YP418724YAN
CSB-EP418724YAN
CSB-BP418724YAN
CSB-MP418724YAN
CSB-EP418724YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L24 (rplX) CSB-YP418725YAN
CSB-EP418725YAN
CSB-BP418725YAN
CSB-MP418725YAN
CSB-EP418725YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L2 (rplB) CSB-YP418726YAN
CSB-EP418726YAN
CSB-BP418726YAN
CSB-MP418726YAN
CSB-EP418726YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 30S ribosomal protein S7 (rpsG) CSB-YP418727YAN
CSB-EP418727YAN
CSB-BP418727YAN
CSB-MP418727YAN
CSB-EP418727YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein tsgA homolog (tsgA), partial CSB-YP418728YAN
CSB-EP418728YAN
CSB-BP418728YAN
CSB-MP418728YAN
CSB-EP418728YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP418729YAN
CSB-EP418729YAN
CSB-BP418729YAN
CSB-MP418729YAN
CSB-EP418729YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP418730YAN
CSB-EP418730YAN
CSB-BP418730YAN
CSB-MP418730YAN
CSB-EP418730YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Xylose isomerase (xylA) CSB-YP418731YAN
CSB-EP418731YAN
CSB-BP418731YAN
CSB-MP418731YAN
CSB-EP418731YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP418732YAN
CSB-EP418732YAN
CSB-BP418732YAN
CSB-MP418732YAN
CSB-EP418732YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Putative reductase YpsIP31758_4159 (YpsIP31758_4159) CSB-YP418733YAN
CSB-EP418733YAN
CSB-BP418733YAN
CSB-MP418733YAN
CSB-EP418733YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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