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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 UPF0133 protein ybaB (ybaB) CSB-YP419474EJD
CSB-EP419474EJD
CSB-BP419474EJD
CSB-MP419474EJD
CSB-EP419474EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Allantoinase (allB) CSB-YP419475EJD
CSB-EP419475EJD
CSB-BP419475EJD
CSB-MP419475EJD
CSB-EP419475EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Citrate lyase acyl carrier protein (citD) CSB-YP419476EJD
CSB-EP419476EJD
CSB-BP419476EJD
CSB-MP419476EJD
CSB-EP419476EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Leucine--tRNA ligase (leuS), partial CSB-YP419477EJD
CSB-EP419477EJD
CSB-BP419477EJD
CSB-MP419477EJD
CSB-EP419477EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0271 protein YbgL (ybgL) CSB-YP419478EJD
CSB-EP419478EJD
CSB-BP419478EJD
CSB-MP419478EJD
CSB-EP419478EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Crossover junction endodeoxyribonuclease rusA (rusA) CSB-YP419479EJD
CSB-EP419479EJD
CSB-BP419479EJD
CSB-MP419479EJD
CSB-EP419479EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP419480EJD
CSB-EP419480EJD
CSB-BP419480EJD
CSB-MP419480EJD
CSB-EP419480EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0145 protein ybjQ (ybjQ) CSB-YP419481EJD
CSB-EP419481EJD
CSB-BP419481EJD
CSB-MP419481EJD
CSB-EP419481EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Asparagine--tRNA ligase (asnS) CSB-YP419482EJD
CSB-EP419482EJD
CSB-BP419482EJD
CSB-MP419482EJD
CSB-EP419482EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Methylglyoxal synthase (mgsA) CSB-YP419483EJD
CSB-EP419483EJD
CSB-BP419483EJD
CSB-MP419483EJD
CSB-EP419483EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 FMN reductase (NADH) RutF CSB-YP419484EJD
CSB-EP419484EJD
CSB-BP419484EJD
CSB-MP419484EJD
CSB-EP419484EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glucans biosynthesis protein G (mdoG) CSB-YP419485EJD
CSB-EP419485EJD
CSB-BP419485EJD
CSB-MP419485EJD
CSB-EP419485EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Flagellar L-ring protein (flgH) CSB-YP419486EJD
CSB-EP419486EJD
CSB-BP419486EJD
CSB-MP419486EJD
CSB-EP419486EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable septum site-determining protein MinC (minC) CSB-YP419487EJD
CSB-EP419487EJD
CSB-BP419487EJD
CSB-MP419487EJD
CSB-EP419487EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Peptidyl-tRNA hydrolase (pth) CSB-YP419488EJD
CSB-EP419488EJD
CSB-BP419488EJD
CSB-MP419488EJD
CSB-EP419488EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Tryptophan synthase alpha chain (trpA) CSB-YP419489EJD
CSB-EP419489EJD
CSB-BP419489EJD
CSB-MP419489EJD
CSB-EP419489EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Zinc transport protein ZntB (zntB), partial CSB-YP419490EJD1
CSB-EP419490EJD1
CSB-BP419490EJD1
CSB-MP419490EJD1
CSB-EP419490EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Autoinducer 2-binding protein lsrB (lsrB) CSB-YP419491EJD
CSB-EP419491EJD
CSB-BP419491EJD
CSB-MP419491EJD
CSB-EP419491EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0060 membrane protein ynfA (ynfA), partial CSB-YP419492EJD1
CSB-EP419492EJD1
CSB-BP419492EJD1
CSB-MP419492EJD1
CSB-EP419492EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA replication terminus site-binding protein (tus) CSB-YP419493EJD
CSB-EP419493EJD
CSB-BP419493EJD
CSB-MP419493EJD
CSB-EP419493EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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