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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Citrobacter koseri Chorismate--pyruvate lyase (ubiC) CSB-YP421156CWS
CSB-EP421156CWS
CSB-BP421156CWS
CSB-MP421156CWS
CSB-EP421156CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP421157CWS
CSB-EP421157CWS
CSB-BP421157CWS
CSB-MP421157CWS
CSB-EP421157CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutamate--cysteine ligase (gshA), partial CSB-YP421158CWS
CSB-EP421158CWS
CSB-BP421158CWS
CSB-MP421158CWS
CSB-EP421158CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP421159CWS
CSB-EP421159CWS
CSB-BP421159CWS
CSB-MP421159CWS
CSB-EP421159CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP421160CWS
CSB-EP421160CWS
CSB-BP421160CWS
CSB-MP421160CWS
CSB-EP421160CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP421161CWS
CSB-EP421161CWS
CSB-BP421161CWS
CSB-MP421161CWS
CSB-EP421161CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Transcriptional repressor rcnR (rcnR) CSB-YP421162CWS
CSB-EP421162CWS
CSB-BP421162CWS
CSB-MP421162CWS
CSB-EP421162CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0235 protein CKO_04329 (CKO_04329) CSB-YP421163CWS
CSB-EP421163CWS
CSB-BP421163CWS
CSB-MP421163CWS
CSB-EP421163CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Undecaprenyl-diphosphatase (uppP) CSB-YP421164CWS
CSB-EP421164CWS
CSB-BP421164CWS
CSB-MP421164CWS
CSB-EP421164CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Urease accessory protein UreG (ureG) CSB-YP421165CWS
CSB-EP421165CWS
CSB-BP421165CWS
CSB-MP421165CWS
CSB-EP421165CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0597 protein CKO_04510 (CKO_04510) CSB-YP421166CWS
CSB-EP421166CWS
CSB-BP421166CWS
CSB-MP421166CWS
CSB-EP421166CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0213 protein CKO_04549 (CKO_04549) CSB-YP421167CWS
CSB-EP421167CWS
CSB-BP421167CWS
CSB-MP421167CWS
CSB-EP421167CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP421168CWS
CSB-EP421168CWS
CSB-BP421168CWS
CSB-MP421168CWS
CSB-EP421168CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP421169CWS1
CSB-EP421169CWS1
CSB-BP421169CWS1
CSB-MP421169CWS1
CSB-EP421169CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP421170CWS
CSB-EP421170CWS
CSB-BP421170CWS
CSB-MP421170CWS
CSB-EP421170CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S8 (rpsH) CSB-YP421171CWS
CSB-EP421171CWS
CSB-BP421171CWS
CSB-MP421171CWS
CSB-EP421171CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 50S ribosomal protein L22 (rplV) CSB-YP421172CWS
CSB-EP421172CWS
CSB-BP421172CWS
CSB-MP421172CWS
CSB-EP421172CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein tsgA homolog (tsgA), partial CSB-YP421173CWS
CSB-EP421173CWS
CSB-BP421173CWS
CSB-MP421173CWS
CSB-EP421173CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri HTH-type transcriptional regulator malT (malT), partial CSB-YP421174CWS
CSB-EP421174CWS
CSB-BP421174CWS
CSB-MP421174CWS
CSB-EP421174CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri DNA ligase B (ligB), partial CSB-YP421175CWS
CSB-EP421175CWS
CSB-BP421175CWS
CSB-MP421175CWS
CSB-EP421175CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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