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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia canadensis Heme A synthase (ctaA), partial CSB-YP423337RMR
CSB-EP423337RMR
CSB-BP423337RMR
CSB-MP423337RMR
CSB-EP423337RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 60 kDa chaperonin (groL), partial CSB-YP423338RMR
CSB-EP423338RMR
CSB-BP423338RMR
CSB-MP423338RMR
CSB-EP423338RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L20 (rplT) CSB-YP423339RMR
CSB-EP423339RMR
CSB-BP423339RMR
CSB-MP423339RMR
CSB-EP423339RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP423340RMR
CSB-EP423340RMR
CSB-BP423340RMR
CSB-MP423340RMR
CSB-EP423340RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP423341RMR
CSB-EP423341RMR
CSB-BP423341RMR
CSB-MP423341RMR
CSB-EP423341RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP423342RMR
CSB-EP423342RMR
CSB-BP423342RMR
CSB-MP423342RMR
CSB-EP423342RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ferredoxin--NADP reductase (A1E_02985) CSB-YP423343RMR
CSB-EP423343RMR
CSB-BP423343RMR
CSB-MP423343RMR
CSB-EP423343RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP423344RMR
CSB-EP423344RMR
CSB-BP423344RMR
CSB-MP423344RMR
CSB-EP423344RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP423345RMR
CSB-EP423345RMR
CSB-BP423345RMR
CSB-MP423345RMR
CSB-EP423345RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP423346RMR
CSB-EP423346RMR
CSB-BP423346RMR
CSB-MP423346RMR
CSB-EP423346RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Putative Holliday junction resolvase (A1E_03830) CSB-YP423347RMR
CSB-EP423347RMR
CSB-BP423347RMR
CSB-MP423347RMR
CSB-EP423347RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP423348RMR
CSB-EP423348RMR
CSB-BP423348RMR
CSB-MP423348RMR
CSB-EP423348RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S5 (rpsE) CSB-YP423349RMR
CSB-EP423349RMR
CSB-BP423349RMR
CSB-MP423349RMR
CSB-EP423349RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S3 (rpsC) CSB-YP423350RMR
CSB-EP423350RMR
CSB-BP423350RMR
CSB-MP423350RMR
CSB-EP423350RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L23 (rplW) CSB-YP423351RMR
CSB-EP423351RMR
CSB-BP423351RMR
CSB-MP423351RMR
CSB-EP423351RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP423352RMR
CSB-EP423352RMR
CSB-BP423352RMR
CSB-MP423352RMR
CSB-EP423352RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis DNA ligase (ligA), partial CSB-YP423353RMR
CSB-EP423353RMR
CSB-BP423353RMR
CSB-MP423353RMR
CSB-EP423353RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Protein RecA (recA) CSB-YP423354RMR
CSB-EP423354RMR
CSB-BP423354RMR
CSB-MP423354RMR
CSB-EP423354RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP423355RMR
CSB-EP423355RMR
CSB-BP423355RMR
CSB-MP423355RMR
CSB-EP423355RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Putative adhesin A1E_05320 (A1E_05320) CSB-YP423356RMR
CSB-EP423356RMR
CSB-BP423356RMR
CSB-MP423356RMR
CSB-EP423356RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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