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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus amyloliquefaciens UPF0302 protein RBAM_020740 (RBAM_020740) CSB-YP423957BQD
CSB-EP423957BQD
CSB-BP423957BQD
CSB-MP423957BQD
CSB-EP423957BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Anthranilate phosphoribosyltransferase (trpD) CSB-YP423958BQD
CSB-EP423958BQD
CSB-BP423958BQD
CSB-MP423958BQD
CSB-EP423958BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP423959BQD
CSB-EP423959BQD
CSB-BP423959BQD
CSB-MP423959BQD
CSB-EP423959BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Pantothenate kinase (coaA) CSB-YP423960BQD
CSB-EP423960BQD
CSB-BP423960BQD
CSB-MP423960BQD
CSB-EP423960BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Bifunctional protein FolD (folD) CSB-YP423961BQD
CSB-EP423961BQD
CSB-BP423961BQD
CSB-MP423961BQD
CSB-EP423961BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L33 1 (rpmG1) CSB-YP423962BQD
CSB-EP423962BQD
CSB-BP423962BQD
CSB-MP423962BQD
CSB-EP423962BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens DNA repair protein recO (recO) CSB-YP423963BQD
CSB-EP423963BQD
CSB-BP423963BQD
CSB-MP423963BQD
CSB-EP423963BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Heat-inducible transcription repressor HrcA (hrcA) CSB-YP423964BQD
CSB-EP423964BQD
CSB-BP423964BQD
CSB-MP423964BQD
CSB-EP423964BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Transcription elongation factor GreA (greA) CSB-YP423965BQD
CSB-EP423965BQD
CSB-BP423965BQD
CSB-MP423965BQD
CSB-EP423965BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Histidine--tRNA ligase (hisS) CSB-YP423966BQD
CSB-EP423966BQD
CSB-BP423966BQD
CSB-MP423966BQD
CSB-EP423966BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L21 (rplU) CSB-YP423967BQD
CSB-EP423967BQD
CSB-BP423967BQD
CSB-MP423967BQD
CSB-EP423967BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glutamyl-tRNA reductase (hemA) CSB-YP423968BQD
CSB-EP423968BQD
CSB-BP423968BQD
CSB-MP423968BQD
CSB-EP423968BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UvrABC system protein C (uvrC), partial CSB-YP423969BQD
CSB-EP423969BQD
CSB-BP423969BQD
CSB-MP423969BQD
CSB-EP423969BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Threonine--tRNA ligase (thrS), partial CSB-YP423970BQD
CSB-EP423970BQD
CSB-BP423970BQD
CSB-MP423970BQD
CSB-EP423970BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0173 metal-dependent hydrolase RBAM_026340 (RBAM_026340) CSB-YP423971BQD
CSB-EP423971BQD
CSB-BP423971BQD
CSB-MP423971BQD
CSB-EP423971BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP423972BQD
CSB-EP423972BQD
CSB-BP423972BQD
CSB-MP423972BQD
CSB-EP423972BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L31 type B (rpmE2) CSB-YP423973BQD
CSB-EP423973BQD
CSB-BP423973BQD
CSB-MP423973BQD
CSB-EP423973BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Diaminopimelate epimerase (dapF) CSB-YP423974BQD
CSB-EP423974BQD
CSB-BP423974BQD
CSB-MP423974BQD
CSB-EP423974BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens FMN-dependent NADH-azoreductase (azoR) CSB-YP423975BQD
CSB-EP423975BQD
CSB-BP423975BQD
CSB-MP423975BQD
CSB-EP423975BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Lactate utilization protein B (lutB) CSB-YP423976BQD
CSB-EP423976BQD
CSB-BP423976BQD
CSB-MP423976BQD
CSB-EP423976BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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