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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia bellii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP423758RIM
CSB-EP423758RIM
CSB-BP423758RIM
CSB-MP423758RIM
CSB-EP423758RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Serine--tRNA ligase (serS) CSB-YP423759RIM
CSB-EP423759RIM
CSB-BP423759RIM
CSB-MP423759RIM
CSB-EP423759RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Putative Na (+)/H (+) antiporter nhaA homolog, partial CSB-YP423760RIM1
CSB-EP423760RIM1
CSB-BP423760RIM1
CSB-MP423760RIM1
CSB-EP423760RIM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Signal peptidase I (lepB), partial CSB-YP423761RIM
CSB-EP423761RIM
CSB-BP423761RIM
CSB-MP423761RIM
CSB-EP423761RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP423762STP
CSB-EP423762STP
CSB-BP423762STP
CSB-MP423762STP
CSB-EP423762STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S10 (rpsJ) CSB-YP423763STP
CSB-EP423763STP
CSB-BP423763STP
CSB-MP423763STP
CSB-EP423763STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S17 (rpsQ) CSB-YP423764STP
CSB-EP423764STP
CSB-BP423764STP
CSB-MP423764STP
CSB-EP423764STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L5 (rplE) CSB-YP423765STP
CSB-EP423765STP
CSB-BP423765STP
CSB-MP423765STP
CSB-EP423765STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L30 (rpmD) CSB-YP423766STP
CSB-EP423766STP
CSB-BP423766STP
CSB-MP423766STP
CSB-EP423766STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana CinA-like protein (Spea_0223) CSB-YP423767STP
CSB-EP423767STP
CSB-BP423767STP
CSB-MP423767STP
CSB-EP423767STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoheptose isomerase (gmhA) CSB-YP423768STP
CSB-EP423768STP
CSB-BP423768STP
CSB-MP423768STP
CSB-EP423768STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana DNA gyrase inhibitor YacG (yacG) CSB-YP423769STP
CSB-EP423769STP
CSB-BP423769STP
CSB-MP423769STP
CSB-EP423769STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana D-ribose pyranase (rbsD) CSB-YP423770STP
CSB-EP423770STP
CSB-BP423770STP
CSB-MP423770STP
CSB-EP423770STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Bifunctional protein HldE (hldE) CSB-YP423771STP
CSB-EP423771STP
CSB-BP423771STP
CSB-MP423771STP
CSB-EP423771STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Sugar fermentation stimulation protein homolog (sfsA) CSB-YP423772STP
CSB-EP423772STP
CSB-BP423772STP
CSB-MP423772STP
CSB-EP423772STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP423773STP
CSB-EP423773STP
CSB-BP423773STP
CSB-MP423773STP
CSB-EP423773STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S16 (rpsP) CSB-YP423774STP
CSB-EP423774STP
CSB-BP423774STP
CSB-MP423774STP
CSB-EP423774STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UPF0246 protein Spea_1078 (Spea_1078) CSB-YP423775STP
CSB-EP423775STP
CSB-BP423775STP
CSB-MP423775STP
CSB-EP423775STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Probable Fe (2+)-trafficking protein CSB-YP423776STP
CSB-EP423776STP
CSB-BP423776STP
CSB-MP423776STP
CSB-EP423776STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP423777STP
CSB-EP423777STP
CSB-BP423777STP
CSB-MP423777STP
CSB-EP423777STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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